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Electrochemical immunosensor for the breast cancer marker CA 15–3 based on the catalytic activity of a CuS/reduced graphene oxide nanocomposite towards the electrooxidation of catechol

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Abstract

The authors report on an electrochemical immunosensor for the tumor marker carbohydrate antigen 15–3 (CA15–3). It is based on the use of a composite consisting of reduced graphene oxide (RGO) and copper sulfide (CuS) that was placed on a screen-printed graphite electrode. The electrode shows excellent activity towards the oxidation of catechol acting as an electrochemical probe, best at a working potential of 0.16 V. The electrode was modified with antibody against CA15–3. Once the analyte (CA15–3) binds to the surface of the electrode, the response to catechol is reduced. The assay has a linear response in the 1.0–150 U mL−1 CA15–3 concentration range, with a 0.3 U mL−1 lower detection limit and a sensitivity of 1.88 μA μM−1 cm−2. The immunosensor also shows good reproducibility (2.7%), stability (95% of the initial values after storing for four weeks). The method was successfully applied to the determination of CA15–3 in serum samples, and results were found to compare well to those obtained by an ELISA. Conceivably, this nanocomposite based detection scheme has a wider scope and may be applied to numerous other immunoassays.

A label-free electrochemical immunosensor based on copper sulfides/graphene nanocomposites was developed for enzyme-free determination of CA15–3 biomarker. This immunosensor can be utilized as a tool to detect of CA15–3 in real samples.

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Acknowledgements

The authors wish to thank the National Institute for Medical Research Development (NIMAD) for their support (Grant No. 957254).

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Authors and Affiliations

  1. Applied Microbiology Research Center, System Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Jafar Amani & Alireza Khoshroo

  2. Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mehdi Rahimi-Nasrabadi

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  1. Jafar Amani
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  2. Alireza Khoshroo
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Correspondence to Mehdi Rahimi-Nasrabadi.

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Amani, J., Khoshroo, A. & Rahimi-Nasrabadi, M. Electrochemical immunosensor for the breast cancer marker CA 15–3 based on the catalytic activity of a CuS/reduced graphene oxide nanocomposite towards the electrooxidation of catechol. Microchim Acta 185, 79 (2018). https://doi.org/10.1007/s00604-017-2532-5

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  • DOI: https://doi.org/10.1007/s00604-017-2532-5

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