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Overlapping-peak resolution and quantification using derivative spectrophotometry in capillary electrophoresis

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Abstract

Derivative spectrophotometry is introduced for the quantification of overlapping peaks in capillary electrophoresis. The application for resolving overlapping peaks is tested using eleven benzoic acids as examples. Compared with common spectrophotometric detection, the derivative mode has slightly lower reproducibility, comparable sensitivity but much higher separation efficiency as the peak is much sharpened. Derivative spectrophotometry offers the advantages of reagent-, time- and labor-saving since only few optimizations of separation conditions for normal CE are essential to make the peaks partly baseline-resolved. Analysis of real samples using the method was also performed and results obtained give evidence of the potential for improved quantification without loss in accuracy.

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Acknowledgements

The financial support from National Natural Science Foundation of China (No.20845002) is gratefully acknowledged.

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Authors and Affiliations

  1. Analytical & Testing Center and College of Chemistry, Sichuan University, Chengdu, 610064, People’s Republic of China

    Jiyou Zhang & Yiyu Hu

  2. School of Physics & Chemistry, Xihua University, Chengdu, 610039, People’s Republic of China

    Jiaqin Liu

  3. College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Zhide Hu

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  1. Jiyou Zhang
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  2. Yiyu Hu
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  3. Jiaqin Liu
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  4. Zhide Hu
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Correspondence to Jiyou Zhang.

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Zhang, J., Hu, Y., Liu, J. et al. Overlapping-peak resolution and quantification using derivative spectrophotometry in capillary electrophoresis. Microchim Acta 164, 487–491 (2009). https://doi.org/10.1007/s00604-008-0088-0

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  • DOI: https://doi.org/10.1007/s00604-008-0088-0

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