Abstract
Purpose
We evaluated the clinical effectiveness of collagen gel droplet-embedded culture drug sensitivity tests (CD-DSTs) in predicting the efficacy of adjuvant chemo-therapeutic treatments for pancreatic cancer (PC).
Methods
The clinicopathological characteristics and prognoses of 22 PC patients who underwent CD-DST after pancreatectomy at Tohoku University between 2012 and 2016 were analyzed retrospectively. Eligibility criteria were resectable or borderline resectable PC, successful evaluation for 5-fluorouracil sensitivity by CD-DST, treatment with S-1 adjuvant chemotherapy, and no preoperative chemotherapy.
Results
The rate of successful evaluation by CD-DST was 52.3% in PC. The optimal T/C ratio, defined as the ratio of the number of cancer cells in the treatment group (T) to that in the control group (C), for 5-fluorouracil was 85% using receiver operating characteristic curve analysis. The sensitive group (T/C ratio < 85%; n = 11) had a better recurrence-free survival rate than the resistant group (T/C ratio ≥ 85%; n = 11; P = 0.029). A Cox proportional hazards regression model demonstrated that sensitivity to 5-fluorouracil was an independent predictor of recurrence on multivariate analysis (hazard ratio 3.28; 95.0% CI 1.20–9.84; P = 0.020).
Conclusions
CD-DSTs helped to predict PC recurrence after S-1 adjuvant chemotherapy.
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Funding
This work was supported in part by a Grant-in-Aid for Scientific Research (24592018) from the Japan Society for the Promotion of Science.
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595_2019_1842_MOESM4_ESM.jpg
Receiver operating characteristic curve of the T/C ratios for predicting recurrence. At the intersection closest to the upper left corner (T/C ratio = 85%), the sensitivity and specificity were 61.1% and 100%, respectively. AUC, area under the curve (JPEG 200 kb)
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Ariake, K., Motoi, F., Mizuma, M. et al. Collagen gel droplet-embedded culture drug sensitivity test (CD-DST) predicts the effect of adjuvant chemotherapy on pancreatic cancer. Surg Today 49, 1035–1043 (2019). https://doi.org/10.1007/s00595-019-01842-5
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DOI: https://doi.org/10.1007/s00595-019-01842-5