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Lack of association between the Pro12Ala polymorphism of the PPAR-γ2 gene and type 2 diabetes mellitus in the Qatari consanguineous population

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Abstract

Peroxisome proliferators-activated receptor γ (PPARγ) is a nuclear hormone receptor that serves as a master regulator for adipocytes-specific genes contributing to adipocytes differentiation, insulin sensitivity and lipid metabolism. The substitution of proline to alanine at codon 12 of the PPAR γ2 gene (Pro12Ala polymorphism) is most widely studied, and the associations with diabetes, obesity, and other clinical parameters have been reported and discussed in several ethnic groups. Among native Qatar ethnicity, however, there is no report about this polymorphism. The aim of this study was to estimate the allele frequency of the Pro12Ala polymorphism of PPAR γ2 gene among Qatari population and investigate the association between this polymorphism and obesity or type 2 diabetes. This is a matched case–control study. It was carried out among diabetic patients and healthy subjects at the Primary Healthcare Clinics, and the survey was conducted from February 2003 to March 2006 in Qatari male and female nationals aged 35 to 60 years. The study was based on matched age, sex, and ethnicity of 400 cases (with diabetes) and 450 controls (without diabetes). Face-to-face interviews were based on a questionnaire that included variables such as age, sex, sociodemographic status, body mass index (BMI), and obesity. Their health status was assessed by medical conditions, family history, and blood pressure measurements. The allele frequency of Pro12Ala polymorphism in PPAR γ2 gene among Qataris is lower than that in many Caucasian ethnic groups. No association is seen between the Pro12Ala and type 2 Diabetes (0.055 vs 0.059, OR = 1.1311, P = 0.669). Nearly half of the diabetic type 2 patients (48.5%) were obese (BMI > 30) compared to nondiabetic subjects (29.8%) (P < 0.001). In this study, no association is seen between the Pro12Ala polymorphism in PPAR γ2 gene and the type2 diabetes in Qatar.

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Acknowledgments

The project was supported and funded by Hamad Medical Corporation (HMC) Grant No. 231. We would like to thank Hamad Medical Corporation and Diabetic Association for generous support and help while this project was conducted. We also thank the technical staff of the Molecular Genetics Laboratory of the HMC for their technical contribution to this study, in particular, Aisha Al-Khelaifi, Ali Alavi, Mashael Al-Jaber, and Irene D’Souza.

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Authors and Affiliations

  1. Molecular Genetics and Lab Medicine and Pathology, Hamad Medical Corporation, Doha, Qatar

    Ramin Badii & Ammar Al-Rikabi

  2. Department of Medical Statistics and Epidemiology, Hamad Medical Corporation, Weill Cornell Medical College Qatar, PO Box 3050, Doha, Qatar

    Abdulbari Bener

  3. Department Evidence for Population Health Unit, School of Epidemiology and Health Sciences, The University of Manchester, Manchester, UK

    Abdulbari Bener

  4. Department of Endocrinology, Hamad Medical Corporation, Hamad General Hospital, Doha, Qatar

    Mahmoud Zirie

  5. Department of Biochemistry, College of Medicine, Sultan Qaboos University, Muscat, Oman

    Mehmet Simsek

  6. Qatar Diabetic Association and Qatar Foundation, Doha, Qatar

    Abdulla O. A. A. Al-Hamaq

  7. CNRS UMR 8090, Institute of Biology of Lille, Pasteur Institute, Lille, France

    Maya Ghoussaini & Philippe Froguel

  8. Medical Research Council Epidemiology Unit, Elsie Widdowson Lab, Cambridge, UK

    Nick J. Wareham

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  1. Ramin Badii
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  2. Abdulbari Bener
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Correspondence to Abdulbari Bener.

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Badii, R., Bener, A., Zirie, M. et al. Lack of association between the Pro12Ala polymorphism of the PPAR-γ2 gene and type 2 diabetes mellitus in the Qatari consanguineous population. Acta Diabetol 45, 15–21 (2008). https://doi.org/10.1007/s00592-007-0013-8

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  • DOI: https://doi.org/10.1007/s00592-007-0013-8

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