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Thrombocytes of diploid and triploid rainbow trouts assessed by flow cytometry and aggregation assay

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Abstract

Thrombocytes are blood cells that protect fish by aggregation to hinder hemorrhages and play a role on pathogen phagocytosis. Moreover, there is a lack of information regarding to the population of thrombocytes and its relationship with fish ploidy. The goal of this work was to determine the thrombocyte numbers and its aggregation capacity in adult diploid and triploid rainbow trouts Oncorhynchus mykiss by flow cytometry screening using monoclonal antibody and magnetic separation. As expected, thrombocytes were in similar proportions between ploidies with 15.26 ± 6.77% in diploid and 13.11 ± 6.37% in triploid blood samples; however, with thrombocytes of triploid trouts showing less aggregation activity when exposed to collagen (85.59 ± 3.36% vs. 95.51 ± 1.25%). In conclusion, triploid thrombocytes have lower aggregation capacity than the diploid ones. This response is possibly related to the deficiency of receptors on the surface of thrombocytes of triploid trouts, which requires further studies for confirmation.

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Authors and Affiliations

  1. Department of Surgery. School of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil

    Renata Stecca Iunes, Lilian de Jesus Oliveira, Janaína Munuera Monteiro, André Luiz Veiga Conrado & José Roberto Kfoury Junior

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  1. Renata Stecca Iunes
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  2. Lilian de Jesus Oliveira
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  3. Janaína Munuera Monteiro
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  4. André Luiz Veiga Conrado
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  5. José Roberto Kfoury Junior
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Correspondence to André Luiz Veiga Conrado.

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Iunes, R.S., de Jesus Oliveira, L., Monteiro, J.M. et al. Thrombocytes of diploid and triploid rainbow trouts assessed by flow cytometry and aggregation assay. Comp Clin Pathol 30, 155–161 (2021). https://doi.org/10.1007/s00580-021-03223-2

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