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Chenopodium quinoa ethanolic extract ameliorates cyclophosphamide®-induced hepatotoxicity in male rats

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Abstract

Cancer is a disease that is associated with abnormal proliferation and growth of living cells, and cyclophosphamide® therapy results in hepatotoxicity. This study aimed to investigate ameliorative effect of Chenopodium quinoa ethanolic extract (QEE) against cyclophosphamide®-induced hepatotoxicity. Four groups (10 male Wistar rats each) were used: (1) healthy control group; (2) rats treated orally with QEE (400 mg/kg/day) for 4 weeks; (3) rats injected intraperitoneally with cyclophosphamide® (150 mg/kg/week) for 4 weeks; (4) rats received QEE after cyclophosphamide® intoxication another 4 weeks. The results revealed that QEE succeeded to decrease the hepatotoxicity-induced by cyclophosphamide®; this was evidenced by the significant reduction in serum ALAT, ASAT, GGT, ALP, total cholesterol, triglycerides, LDL-cholesterol, and TNF-α IL 1β and IL6, as well as hepatic MDA, nitric oxide levels, and DNA fragmentation coupled with a marked rise in serum albumin and HDL-cholesterol level as well as hepatic GSH, SOD, GPx, and catalase values. QEE succeeded also in improving the histopathological picture of the liver. It could be concluded that QEE succeeded, to a great extent, to counteract the oxidative stress and regenerated the liver against cyclophosphamide®-resultant hepatotoxicity. QEE could be considered a promising candidate as a food supplement for the protection against the side effects of cyclophosphamide®.

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Authors and Affiliations

  1. Medical Physiology Department, National Research Centre, Giza, Egypt

    Khaled G. Abdel-Wahhab & Fathia A. Mannaa

  2. Zoology Department, Faculty of Science, Al-Azhar University, Asyut, Egypt

    Mahmoud Ashry

  3. Histology Department, Faculty of Medicine, Helwan University, Cairo, Egypt

    Doaa M. Khaled

  4. Dairy Department, National Research Centre, Giza, Egypt

    Laila K. Hassan

  5. Biological Sciences Department, Faculty of Sciences and Arts, Scientific Department, Qassium University, Buraydah, Saudi Arabia

    Heba F. Gomaa

  6. Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

    Heba F. Gomaa

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  1. Khaled G. Abdel-Wahhab
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  2. Fathia A. Mannaa
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  3. Mahmoud Ashry
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Correspondence to Khaled G. Abdel-Wahhab.

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All animals received human care in accordance with the standard institutional criteria for the care and use of experimental animals according to the Ethical Committee of the National Research Center (FWA 00014747).

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Abdel-Wahhab, K.G., Mannaa, F.A., Ashry, M. et al. Chenopodium quinoa ethanolic extract ameliorates cyclophosphamide®-induced hepatotoxicity in male rats. Comp Clin Pathol 30, 267–276 (2021). https://doi.org/10.1007/s00580-021-03199-z

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