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Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch

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Abstract

The purpose of this study was to assess the hypoglycemic and protective activities of the ethanolic extract of Allium saralicum (AS) in streptozotocin (STZ) induced diabetic mice. Diabetes was experimentally induced by intraperitoneal injection of STZ (60 mg/kg bw) in 35 mice. Fasting blood glucose (FBG) levels were measured everyday using glucometer strips. Mice with plasma glucose level > 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into five groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 0.5 mg/kg glibenclamide orally. Groups 4 and 5 were given 200 and 400 μg/kg, respectively, of AS for 15 days orally. At 16th day, the animals were dissected and their kidney removed. The total volume of cortex, medulla, renal tubules, and glomeruli as well as the length of vessels and renal tubules was estimated using stereological method. Daily treatment of diabetic mice with high dose of AS significantly (p ≤ 0.05) declined FBG levels at days 10–13 of the experiment faster than glibenclamide. High dose of AS could significantly (p ≤ 0.05) improve the volume of cortex, glomeruli, proximal tubules, distal tubules, vessels, and total length of proximal tubules and vessels. Moreover, glomerular loss was significantly (p ≤ 0.05) inhibited with high dose of AS. It concluded that Allium saralicum could improve hyperglycemia and ameliorated renal changes following diabetes in STZ-induced diabetic mice. So, according to these features, it could be used as an antidiabetic supplement.

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh & Akram Zangeneh

  2. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Akram Zangeneh & Reza Tahvilian

  3. Department of Basic and Pathobiological Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Nader Goodarzi

  4. Department of Dermatology, School of Medicine, Kermanshah University of Medical Science, Kermanshah, Iran

    Fariba Najafi

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  1. Mohammad Mahdi Zangeneh
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  2. Nader Goodarzi
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  3. Akram Zangeneh
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  4. Reza Tahvilian
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  5. Fariba Najafi
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Correspondence to Nader Goodarzi.

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All institutional and national standards for the care and use of laboratory animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Zangeneh, M.M., Goodarzi, N., Zangeneh, A. et al. Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol 27, 861–867 (2018). https://doi.org/10.1007/s00580-018-2674-9

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  • DOI: https://doi.org/10.1007/s00580-018-2674-9

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