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Hemato-immunological and plasma biochemical responses of silvery-black porgy (Sparidentex hasta) fed protein and essential amino acid deficient diets

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Abstract

A 6-week feeding trial was conducted to evaluate the effects of protein free (PF) and essential amino acid deficient (EAAD) diets on the physiological responses of silvery-black porgy (Sparidentex hasta) juveniles. Three experimental diets were formulated: a control diet in which 60% of dietary nitrogen was provided by intact protein (fish meal) and 40% by crystalline AA [(blends of essential amino acids (EAAs) and non-essential amino acids (NEAAs)]; an essential amino acid deficient diet in which 60% of dietary N was provided by intact protein, whereas the rest was provided by NEAAs; and a protein free (PF) diet, which based on carbohydrate sources. Fish fed the PF and EAAD showed signs of anemia including lower red blood cells counts, hemoglobin, and hematocrit levels than control group. Plasma lysozyme activity and complements C3 and C4, as well as total immunoglobulin levels were drastically reduced in fish fed PF and EAAD diets. Plasma and liver alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase were significantly increased, but superoxide dismutase was decreased in fish fed PF and EAAD diets. Plasma total protein, albumin, high density lipoprotein, calcium, and inorganic phosphorous significantly decreased in fish fed PF and EAAD diets. The information obtained from this study testing to extreme diets (EAAD and PF diets) may serve for better understanding the impact of protein nutritional imbalances in fish.

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Acknowledgements

We are grateful to the director and staff of the Mariculture Research Station, Sarbandar, Iran for providing the necessary facilities for the experiment.

Funding

This study was funded by Iran National Science Foundation (grant number 92011610).

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Authors and Affiliations

  1. Agriculture Research, Education and Extension, Iran Fisheries Research Organization (IFRO), South Iran Aquaculture Research Center, Ahwaz, Iran

    Mansour Torfi Mozanzadeh, Morteza Yaghoubi & Jasem G. Marammazi

  2. Department of Fisheries, Faculty of Natural Resources and Environment, Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi, Iran

    Omid Safari

  3. IRTA, Centre de Sant Carles de la Rápita (IRTA-SCR), Unitat de Cultius Experimentals, Crta. del Poble Nou Km 5.5, Sant Carles de la Rápita, Spain

    Enric Gisbert

Authors
  1. Mansour Torfi Mozanzadeh
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  2. Morteza Yaghoubi
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  3. Jasem G. Marammazi
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  4. Omid Safari
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  5. Enric Gisbert
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Correspondence to Mansour Torfi Mozanzadeh.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Mozanzadeh, M.T., Yaghoubi, M., Marammazi, J.G. et al. Hemato-immunological and plasma biochemical responses of silvery-black porgy (Sparidentex hasta) fed protein and essential amino acid deficient diets. Comp Clin Pathol 27, 55–60 (2018). https://doi.org/10.1007/s00580-017-2551-y

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  • DOI: https://doi.org/10.1007/s00580-017-2551-y

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