Skip to main content
SpringerLink
Log in

Short- and long-term exposure to imidacloprid disturbs the elemental composition and free amino acid profile in muscles of Labeo rohita

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitro-imidazolidin-2-ylideneamine] is among the most extensively used pesticide in Pakistan. The aim of this investigation was to report the changes in concentration of different elements and amino acid in the muscle of Labeo rohita upon imidacloprid exposure. Juvenile Labeo rohita (N = 480) were treated with 120mgL−1 imidacloprid under short and long-term experimental conditions. At the end of each experiment, fish muscle was used for elemental concentration (determination by an atomic absorption spectrophotometer) and amino acid estimation (by high-performance liquid chromatography (HPLC)). Our results indicated that sodium, potassium, calcium, magnesium, iron, aluminum, and lead concentrations were significantly different (P < 0.05) when compared between imidacloprid-treated and untreated L. rohita and changes were more pronounced in short-term experimental groups. HPLC-generated data analysis showed that proline, isoleucine, and glycine concentrations increased while the levels of phenylalanine, alanine, and leucine decreased significantly (P < 0.05) in muscles of fish exposed to imidacloprid under short-term conditions. On the other hand, serine, arginine, and glycine increased significantly and alanine decreased significantly in L. rohita exposed to pesticide under long-term experimental conditions. It is concluded that imidacloprid (120 mg L−1) exposure to L. rohita for a variable duration has significantly changed the elemental composition and studied amino acid concentrations in fish muscles making it unsuitable for human consumption.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anita ST, Sobha K, Veeraiah K et al (2010) Studies on biochemical changes in the tissues of Labeo rohita and Cirrhinus mrigala exposed to fenvalerate technical grade. J Toxicol Environ Health Sci 2(5):53–62

    Google Scholar 

  • Arora P, Sharma H (2012) Effect of insecticide such as cypermethrin on protein content, mortality and cod liver oil concentration of fish Labeo rohita. Int J Res Eng Agricul Sci 2(11):7–20

    CAS  Google Scholar 

  • Bansal SK, Verma SR, Gupta AK et al (1979) Physiological dysfunction of the haemopoietic system in a freshwater teleost Labeo rohita following chronic chlordane exposure. Part I. Alteration in certain hematological parameters. Bull Environ Contam Toxicol 22:666–673

    Article  CAS  PubMed  Google Scholar 

  • Canli M, Atli G (2002) The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species. Environ Pollut 121:129–136

    Article  Google Scholar 

  • Durga P, Veeraiah K (2002) Effect of cypermethrin on protein metabolism of the fish, Labeo rohita (Hamilton). Bull Pure Appl Sci 21 A(1):27–32

    Google Scholar 

  • Dzhikiia GM, Mchedluri TT, Nikolaishvili MI et al (2011) The pyretroind pesticides lambda-cigalothrines influence on composition of free amino acids in tissue of fish in Alazani River. Georg Med News 201:52–55

    Google Scholar 

  • Exley C, Chappel JS, Birchall JD (1991) A mechanism for acute aluminium toxicity to fish. J Theor Biol 151:417–428

    Article  CAS  PubMed  Google Scholar 

  • Grosell MH, Hogstrand C, Wood CM (1997) Cu uptake and turnover in both Cu acclimated and non-acclimated rainbow trout (Oncorhychus mykiss). Aqua Toxicol 38:257–276

    Article  CAS  Google Scholar 

  • Li X, Ping X, Xiumei S et al (2005) Toxicity of cypermethrin on growth, pigments, and superoxide dismutase of Scenedesmus obliquus. Ecotoxcol Environ Safe 60(2):188–192

    Article  CAS  Google Scholar 

  • Matsuda K, Buckingham SD, Kleier D et al (2001) Neonicotinoids: insecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol Sci 22:573–580

    Article  CAS  PubMed  Google Scholar 

  • Moza PN, Hustert K, Feicht E, Kettrup A (1998) Photolysis of imidacloprid in aqueous solution. Chemosphe 36(3):497–502

    Article  CAS  Google Scholar 

  • Muhammad F, Javed I, Akhtar M et al (2012) Quantitative structure activity relationship and risk analysis of some pesticides in the cattle milk. Pak Vet J 32:589–592

    CAS  Google Scholar 

  • Naeem M, Salam A, Tahir SS et al (2011) The effect of fish size and condition on the contents of twelve essential and non essential elements in Aristichthys nobilis from Pakistan. Pak Vet J 31:109–112

    CAS  Google Scholar 

  • Naz S, Javed M (2012) Acute toxicity of metals mixtures for fish, Catla catla, Labeo rohita and Cirrhina mrigala. Pak J Agric Sci 49:387–391

    Google Scholar 

  • Ogino C, Yang GY (1980) Requirements of carp and rainbow trout for dietary manganese and cooper. Bull Jap Soc Sci Fish 46:455–458

    Article  CAS  Google Scholar 

  • PMRA (Pest Management Regulatory Agency) (2005). Electronic dossier, delivery, and evaluationsystem (EDDE): Electronic Labels: Search and Evaluation (ELSE).http://eddenet.pmra-arla.gc.ca/4.0/4.01

  • Polat H, Erkoc FU, Viran R et al (2002) Investigation of acute toxicity of β-cypermethrin on Guppies Poecilia reticulate. Chemosphere 49(1):39–44

    Article  CAS  PubMed  Google Scholar 

  • Prashanth MS, David M (2006) Changes in nitrogen metabolism of the freshwater fish Cirrhinus mrigala following exposure to cypermethrin. J Basic Clin Physiol Pharmacol 17(1):63–70

    Article  CAS  PubMed  Google Scholar 

  • Qadir S, Iqbal F (2016) Effect of sublethal concentration of imidacloprid on the histology of heart, liver and kidney in Labeo rohita. Pak J Pharmaceut Sci 29(6):2033–2038

    Google Scholar 

  • Qadir S, Latif A, Ali M et al (2014) Effects of imidacloprid on the hematological and serum biochemical profile of Labeo rohita. Pak J Zool 46(4):1085–1090

    CAS  Google Scholar 

  • Qadir S, Bukhari R, Iqbal F (2015) Effect of sub lethal concentration of imidacloprid, on proximate body composition of Labeo rohita. Iran J Fish Sci 14(4):937–945

    Google Scholar 

  • Saravanan TS, Rajesh P, Sundaramoorthy M (2010) Studies on effect of chronic exposure of endosulfan to Labeo rohita. J Environ Biol 31(5):755–758

    CAS  Google Scholar 

  • Sarkar M, Biswas P, Roy S, Kole R, Chowdhury A (1999) Effect of pH and type of formulation on the persistence of imidacloprid in water. Bull Environ Contam Toxicol 63(5):604–609

    Article  CAS  PubMed  Google Scholar 

  • Schulz R (2004) Field studies on exposure effects and risk mitigation of aquatic nonpoint-source insecticide pollution: a review. J Environ Qualt 33:419–448

    Article  CAS  Google Scholar 

  • Singh NN, Das VK, Shrivastava AK (2002) Insecticides and ionic regulation in teleosts: a review. Zoologica Poloniae 47(3–4):21–36

    Google Scholar 

  • Singh KS, Singh SKS, Yadav RP (2010) Toxicological and biochemical alterations of cypermethrin (synthetic pyrethroids) against freshwater teleost fish Colisa fasciatus at different seasons. World J Zool 5(1):25–32

    Google Scholar 

  • Thenmozhi C, Vignesh V, Thirumurugan R et al (2011) Impacts of malathion on mortality and biochemical changes of freshwater fish Labeo rohita. Iran J Environ Health Sci Eng 8:4–7

    Google Scholar 

  • Tomizawa M, Casida JE (2005) Neonicotinoid insecticide toxicology: mechanisms of selective action. Ann Rev Pharmacol Toxicol 45:247–268

    Article  CAS  Google Scholar 

  • Toth T, Andreji J, Toth J et al (2012) Cadmium lead and mercury contents in fishes—case study. J Micro Biotech Food Sci 1:837–847

    CAS  Google Scholar 

  • Wamhoff H, Schneider V (1999) Photodegradation of imidacloprid. J Agri Food Chem 47(4):1730–1734

    Article  CAS  Google Scholar 

  • Yousaf M, Salam A, Naeem M et al (2012) Effect of body size on elemental concentration in wild Wallago attu (Bloch and Schneider) from southern Punjab, Pakistan. Afr J Biotech 11(7):1764–1176

    CAS  Google Scholar 

  • Zheng W, Liu W (1999) Kinetics and mechanism of the hydrolysis of imidacloprid. Pest Sci 55(4):482–485

    Article  CAS  Google Scholar 

Download references

Funding

The authors are grateful to Higher Education Commission (HEC) of Pakistan for providing research grant for this study under Indigenous PhD Scholarship Scheme.

Author information

Authors and Affiliations

  1. Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Shazia Qadir & Furhan Iqbal

  2. Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310058, China

    Chen Xiwei, Wei Wei & Fengqin Feng

  3. Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China

    Chen Xiwei, Wei Wei & Fengqin Feng

  4. Department of Chemistry, School of Science, Xi’an Jiaotong University, Xi’an, 710049, China

    Shahid Sultan & Khalida Kareem

  5. Department of Zoology, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan

    Furhan Iqbal

Authors
  1. Shazia Qadir
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chen Xiwei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fengqin Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shahid Sultan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Khalida Kareem
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Furhan Iqbal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Furhan Iqbal.

Ethics declarations

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All the experimental procedure and fish handling protocols were approved by the Ethical Committee of Zoology Department.

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Qadir, S., Xiwei, C., Wei, W. et al. Short- and long-term exposure to imidacloprid disturbs the elemental composition and free amino acid profile in muscles of Labeo rohita . Comp Clin Pathol 26, 1339–1346 (2017). https://doi.org/10.1007/s00580-017-2538-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-017-2538-8

Keywords

Search

Navigation