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Detection of paratuberculosis using histopathology, immunohistochemistry, and ELISA in West Algeria

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Abstract

Paratuberculosis (Ptb), caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic enteritis that affects many ruminants and other wild animals worldwide. Although the prevalence of infection is unknown in nearly all countries, many studies report that paratuberculosis is the greatest economic problem in animal health. Here, we report the occurrence of paratuberculosis in herds of cattle in the Tiaret region of Algeria, using different diagnostic procedures. Tiaret and Sougueur abattoirs were being selected representing the main parts of the province. Four hundred intestines were examined from apparently healthy cattle that were slaughtered at Tiaret city (300) and Sougueur (100) slaughterhouses. Occurrence of subclinical paratuberculosis in cattle was 54, 51, 8, and 7.5 % using histopathology, IHC, ELISA, and ZN techniques, respectively. Histopathology is reported to be a better diagnostic tool mainly when complemented with immunohistochemistry.

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Acknowledgments

We thank Dr. WAEL Hannaneh for his help and advice.

Conflict of interest

Authors have nothing to disclose.

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Authors and Affiliations

  1. Institute of Veterinary Sciences, Ibn-Khaldoun University, BP P 78 zaâroura, 14000, Tiaret, Algeria

    H. Hemida

  2. Laboratory of Applied Microbiology, Department of Biology, Faculty of Science, Oran University, BP 16, Es-senia, 31100, Oran, Algeria

    M. Kihal

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  1. H. Hemida
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Correspondence to H. Hemida.

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Hemida, H., Kihal, M. Detection of paratuberculosis using histopathology, immunohistochemistry, and ELISA in West Algeria. Comp Clin Pathol 24, 1621–1629 (2015). https://doi.org/10.1007/s00580-015-2126-8

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  • DOI: https://doi.org/10.1007/s00580-015-2126-8

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