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Apoptotic and cytotoxic effect of Semecarpus anacardium Linn nut milk extract on MCF-7 breast cancer cells

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Abstract

Semecarpus anacardium Linn nut milk extract (SA) has been used in Siddha system of medicine against various ailments. The present study was designed to evaluate the apoptotic and cytoprotective effect of the drug SA on MCF-7 breast cancer cell line. The effect of the drug SA on growth rates of MCF-7 cells were determined by trypan blue exclusion method whereas cytotoxicity was determined by lactate dehydrogenase (LDH) assay. In order to determine whether the drug-mediated cell death of cancer cells was either through apoptosis or necrosis, PI staining was done. We observed that the drug SA exhibited cytotoxic effect and inhibited the growth and proliferation of MCF-7 cells through apoptosis compared to untreated control cells in a dose-dependent manner. These results confirm that the cytotoxicity that was mediated was different depending on the concentration of the nut extract used.

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References

  • Abu-Dahab R, Afifi F (2007) Antiproliferative activity of selected medicinal plants of Jordan against a breast adenocarcinoma cell line (MCF-7). Sci Pharm 75:121–136

    Article  Google Scholar 

  • Aravind SG, Arimboor R, Rangan M, Madhavan SN, Arumughan C (2008) Semi-preparative HPLC preparation and HPTLC quantification of tetrahydroamentoflavone as marker in Semecarpus anacardium and its polyherbal formulations. J Pharm Biomed Anal 48:808–813

    Article  CAS  PubMed  Google Scholar 

  • Arulvasu C, Prabhu D, Manikandan R, Srinivasan P, Dinesh D, Babu G, Sellamuthu S (2010) Induction of apoptosis by the aqueous and ethanolic leaf extract of Vitex negundo L. in MCF-7 human breast cancer cells. Int J Drug Disc 2:01–07

    Google Scholar 

  • Chiruvella KK, Kari V, Choudhary B, Nambiar M, Ghanta RG et al (2008) Methyl angolensate, a natural tetranortriterpenoid induces intrinsic apoptotic pathway in leukemic cells. FEBS Lett 582:4066–4076

    Article  CAS  PubMed  Google Scholar 

  • Dhanasekaran SM, Jaganathan R, Panchanadham S, Palanivelu S (2012) Induction of mitochondrial mediated apoptosis by Semecarpus anacardium in the BCR-ABL+ 12B1 leukemia cell line—a possible mechanism of therapeutic action in vivo. J Exp Clin Med 4(1):30–38

    Article  CAS  Google Scholar 

  • Ferlay JSH, Bray F, Forman D, Mathers C, Parkin DM (2010) GLOBOCAN 2008 v1.2, Cancer Incidence and Mortality Worldwide: IARC Cancer Base 10

  • Formulary of Siddha Medicine (1972) 2nd edn. Madras: Indian Medicine Practitioners Co-operative Pharmacy and Stores Ltd: 197

  • Haseena Banu H, Kaladevi S, Ashwini S, Shanthi P, Sachdanandam P (2011) Antidiabetic and antioxidant effect of Semecarpus anacardium Linn nut milk extract in a high fat diet stz induced type 2 diabetic rat model. Comp Clin Pathol 23(4):875–884

  • Haseena Banu H, Kaladevi S, Kumar S, Shanthi P, Sachdanandam P (2013) Antihypercholesterolemic effect of Semecarpus anacardium in high cholesterol fed rats. Comp Clin Pathol. doi:10.1007/s00580-013-1706-8

    Google Scholar 

  • Hu W, Kavanagh JJ (2003) Anticancer therapy targeting the apoptotic pathway. Lancet Oncol 4:721–9

  • Khan HBH, Vinayagam KS, Madan P, Palanivelu S, Panchanadham S (2011) Modulatory effect of Semecarpus anacardium against oxidative damages in DMBA induced mammary carcinogenesis rat model. Comp Clin Pathol 21:1275–1284

    Article  Google Scholar 

  • Koppikar SJ, Choudhari AS, Suryavanshi SA, Kumari S, Chattopadhyay S, Kaul-ghanekar R (2010) Aqueous cinnamon extract(ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell lines (SiHa) through loss of mitochondrial membrane potential. BMC Cancer. doi:10.1186/1471-2407-10-210

    PubMed Central  PubMed  Google Scholar 

  • Leong SP, Shen ZZ, Liu T-J, Agarwal G, Tajima T, Paik N-S, Sandelin K, Derossis A, Cody H, Foulkes WD (2010) Is breast cancer the same disease in Asian and Western countries? World J Surg 34:2308–2324

    Article  PubMed Central  PubMed  Google Scholar 

  • Maganha EG, da Costa Halmenschlager R, Rosa RM, Henriques JAP, de Paula Ramos ALL, Jenifer S (2010) Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus. Food Chem 118:1–10

    Article  CAS  Google Scholar 

  • Marsoni S, Damia G (2004) Molecular targeting: new therapeutic strategies to improve tumour apoptosis. Ann Oncol 15(4):229–231

    Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63

    Article  CAS  PubMed  Google Scholar 

  • Nair PKR, Melnick SJ, Wnuk SF, Rapp M, Escalon E, Ramachandran C (2009) Isolation and characterization of an anticancer catechol compound from Semecarpus anacardium. J Ethnopharmacol 122:450–456

    Article  CAS  PubMed  Google Scholar 

  • Premalatha B, Muthulakshmi V, Vijayalakshmi T, Sachdanandam P (1997) Semecarpus anacardium nut extract induced changes in enzymic antioxidants studied in aflatoxin B1 caused hepatocellular carcinoma bearing Wistar rats. Int J Pharm 3:161–166

    Article  Google Scholar 

  • Ramprasath VR, Shanthi P, Sachdanandam P (2006) Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium Linn. Nut milk extract in experimental inflammatory conditions. Biol Pharm Bull 29:693–700

    Article  CAS  PubMed  Google Scholar 

  • Rubinstein LV, Shoemaker RH, Paull KD, Simon RM, Tosini S, Skehan P, Scudiero DA, Monks A, Boyd MR (1990) Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J Natl Cancer I 82:1113–1118

  • Shahabuddin MS, Nambiar M, Moorthy BT, Naik PL, Choudhary B et al (2011) A novel structural derivative of natural alkaloid ellipticine, MDPSQ, induces necrosis in leukemic cells. Investig New Drugs 29:523–533

    Article  CAS  Google Scholar 

  • Shin YG, Cordell GA, Dong Y, Pezzuto JM, Appa Rao AVN, Ramesh M, Ravi Kumar B, Radhakishan M (1999) Rapid identification of cytotoxic alkenyl catechols in Semecarpus anacardium using bioassay-linked high performance liquid chromatography-electrospray/mass spectrometric analysis. Phytochem Anal 10:208–212

    Article  CAS  Google Scholar 

  • Suganthi S, Haseena Banu H, Shanthi P, Sachdanandam P (2014) Antihyperlipidemic and antiinflammatory effect of Bhallataka nuts in ameliorating the alterations in lipid metabolism and inflammation in diabetes induced cardiac damage in rats. Comp Clin Pathol 23(5):1593–1601

    Article  Google Scholar 

  • Taraphdar AK, Roy M, Bhattacharya RK (2001) Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Current Sci. 80:10–11

  • Vladimir J, Ivan S, Gordana K (1999) A comparison of the NK cell cytotoxicity with effects of TNF- α against K-562 cells, determined by LDH release assay. Cancer Lett 138:67–72

    Article  Google Scholar 

  • Wu P, Tarasenko YI, Gu Y, Huang LY, Coggeshall RE, Yu Y (2002) Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat. Nat Neurosci 5:1271–1278

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Acknowledgments

Financial assistance from the ICMR Adhoc project in the form of Senior Research Fellowship, New Delhi, is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Pathology, University of Madras, Taramani Campus, Chennai, 600113, India

    Haseena Banu Hedayathullah Khan & Shanthi Palanivelu

  2. Department of Medical Biochemistry, University of Madras, Taramani Campus, Chennai, 600113, India

    Sachidanandam Thiruvaiyaru Panchanadham

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  1. Haseena Banu Hedayathullah Khan
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  2. Sachidanandam Thiruvaiyaru Panchanadham
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  3. Shanthi Palanivelu
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Correspondence to Shanthi Palanivelu.

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Khan, H.B.H., Panchanadham, S.T. & Palanivelu, S. Apoptotic and cytotoxic effect of Semecarpus anacardium Linn nut milk extract on MCF-7 breast cancer cells. Comp Clin Pathol 24, 1439–1444 (2015). https://doi.org/10.1007/s00580-015-2092-1

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