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Tracing Rhizophagus irregularis isolate IR27 in Ziziphus mauritiana roots under field conditions

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A Correction to this article was published on 01 January 2020

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Abstract

Arbuscular mycorrhizal fungi (AMF) play a major role as biofertilizer for sustainable agriculture. Nevertheless, it is still poorly documented whether inoculated AMF can successfully establish in field soils as exotic AMF and improve plant growth and productivity. Further, the fate of an exogenous inoculum is still poorly understood. Here, we pre-inoculated two cultivars (Tasset and Gola) of the fruit tree Ziziphus mauritiana (jujube) with the exotic AM fungus Rhizophagus irregularis isolate IR27 before transplantation in the field. In two experiments, tracking and quantification of R. irregularis IR27 were assessed in a 13-month-old jujube and an 18-month-old jujube in two fields located in Senegal. Our results showed that the inoculant R. irregularis IR27 was quantitatively traced and discriminated from native R. irregularis isolates in roots by using a qPCR assay targeting a fragment of the RNA polymerase II gene (RPB1), and that the inoculum represented only fractions ranging from 11 to 15% of the Rhizophagus genus in the two plantations 13 and 18 months after transplantation, respectively. This study validates the use of the RPB1 gene as marker for a relative quantification of a mycorrhizal inoculant fungus isolate in the field.

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Change history

  • 06 February 2020

    The authors of the above-mentioned published article inadvertently omitted Dirk Redecker, Dioumacor Fall and Diaminatou Sanogo from the list of authors. The names and their affiliations presented in this paper.

  • 06 February 2020

    The authors of the above-mentioned published article inadvertently omitted Dirk Redecker, Dioumacor Fall and Diaminatou Sanogo from the list of authors. The names and their affiliations presented in this paper.

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Funding

This work was funded by the Africa-Brazil-France tripartite research program entitled “Fight against desertification in Africa” (2014–2016). Babacar Thioye received grants from the Institut de Recherche pour le Développement (IRD), the Institut Sénégalais de Recherches Agricoles (ISRA), and from the French Embassy in Senegal (SCAC).

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Authors and Affiliations

  1. Laboratoire Commun de Microbiologie IRD/ISRA/UCAD, BP 1386, Dakar, Senegal

    Babacar Thioye, Aboubacry Kane, Cheikh Ndiaye & Samba Ndao Sylla

  2. Laboratoire des Symbioses Tropicales et Méditerranéennes UMR113 INRA/AGRO-M/CIRAD/IRD/UM2-TA10/J, Campus International de Baillarguet, 34398, Montpellier, France

    Babacar Thioye, Robin Duponnois & Amadou Mustapha Bâ

  3. Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000, Dijon, France

    Diederik van Tuinen

  4. Embrapa Agrobiologia, Km 7 BR 465, Seropedica, Rio de Janeiro, 23890 000, Brazil

    Sergio Mania de Faria

  5. Laboratoire de Biologie et Physiologie Végétales, Faculté des Sciences Exactes et Naturelles, Université des Antilles, BP 592, 97159, Pointe-à-Pitre, Guadeloupe, France

    Amadou Mustapha Bâ

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  1. Babacar Thioye
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  2. Diederik van Tuinen
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Correspondence to Babacar Thioye.

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Thioye, B., van Tuinen, D., Kane, A. et al. Tracing Rhizophagus irregularis isolate IR27 in Ziziphus mauritiana roots under field conditions. Mycorrhiza 29, 77–83 (2019). https://doi.org/10.1007/s00572-018-0875-3

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