Abstract
Many different cultivation techniques and inoculum products of the plant-beneficial arbuscular mycorrhizal (AM) fungi have been developed in the last decades. Soil- and substrate-based production techniques as well as substrate-free culture techniques (hydroponics and aeroponics) and in vitro cultivation methods have all been attempted for the large-scale production of AM fungi. In this review, we describe the principal in vivo and in vitro production methods that have been developed so far. We present the parameters that are critical for optimal production, discuss the advantages and disadvantages of the methods, and highlight their most probable sectors of application.
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Acknowledgements
This research was funded by a Marie Curie Early-Stage Research Training Fellowship of the European Community’s Sixth Framework Program, under contract number MEST CT-2005-021016. S.C. acknowledges the financial support from the Belgian Federal Office for Scientific, Technical, and Cultural affairs under contract number C4/00/001.
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IJdo, M., Cranenbrouck, S. & Declerck, S. Methods for large-scale production of AM fungi: past, present, and future. Mycorrhiza 21, 1–16 (2011). https://doi.org/10.1007/s00572-010-0337-z
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DOI: https://doi.org/10.1007/s00572-010-0337-z