Abstract
A study was conducted to define culture conditions for in vitro growth arbuscular mycorrhizal fungi (AMF) with liverworts as hosts. Lunularia cruciata (L.) Dumortier ex. Lindberg developed in vitro monoxenic mycothalli with both Glomus proliferum Dalpé & Declerck (MUCL 41827) and Glomus intraradices Schenck & Smith (MUCL 43204). AMF inoculated plants were co-cultured in plastic Petri dishes with semi-solidified medium supplemented with sucrose and grown under filtered light. Mycothalli of L. cruciata produced external hyphae and spores in quantities equivalent to those obtained with Ri T-DNA transformed root systems.
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Acknowledgements
We would like to thank Dr. Manuel Santos for the use of imaging facilities at the Laboratory of Molecular Genomics, Dr. Edgar da Cruz e Silva for critically reading and making valuable suggestions on the manuscript, Dr. Manuel J. A. P. Soares for the use of Spectroscopy Laboratory FSCOSD, Department of Physics and Mr Ricardo Ribeiro and fellow students for their participation on preliminary studies with liverworts. Ms Hélia Costa provided technical support in sample preparation for light microscopy. Dr. Ricardo L. L. Berbara has a fellowship grant from CNPq–Brazil.
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Fonseca, H.M.A.C., Berbara, R.L.L. & Pereira, M.L. Lunularia cruciata, a potential in vitro host for Glomus proliferum and G. intraradices . Mycorrhiza 16, 503–508 (2006). https://doi.org/10.1007/s00572-006-0061-x
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DOI: https://doi.org/10.1007/s00572-006-0061-x