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Interactions between the arbuscular mycorrhizal (AM) fungus Glomus intraradices and nontransformed tomato roots of either wild-type or AM-defective phenotypes in monoxenic cultures

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Abstract

Monoxenic symbioses between the arbuscular mycorrhizal (AM) fungus Glomus intraradices and two nontransformed tomato root organ cultures (ROCs) were established. Wild-type tomato ROC from cultivar “RioGrande 76R” was employed as a control for mycorrhizal colonization and compared with its mutant line (rmc), which exhibits a highly reduced mycorrhizal colonization (rmc) phenotype. Structural features of the two root lines were similar when grown either in soil or under in vitro conditions, indicating that neither monoxenic culturing nor the rmc mutation affected root development or behavior. Colonization by G. intraradices in monoxenic culture of the wild-type line was low (<10%) but supported extensive development of extraradical mycelium, branched absorbing structures, and spores. The reduced colonization of rmc under monoxenic conditions (0.6%) was similar to that observed previously in soil. Extraradical development of runner hyphae was low and proportional to internal colonization. Few spores were produced. These results might suggest that carbon transfer may be modified in the rmc mutant. Our results support the usefulness of monoxenically obtained mycorrhizas for investigation of AM colonization and intraradical symbiotic functioning.

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Acknowledgements

This work was supported by an OECD (Co-operative Research Programme: Biological Resource Management for Sustainable Agriculture Systems) fellowship to AB, which enabled his visit to Adelaide, and by the Spanish Ministry of Science and Research (Ref. AGL2001-1363). CC was partially supported by Project REN2003-00968GLO (MEC; JM Barea, I. P.). SD was in receipt of a postdoctoral research fellowship from the Australian Research Council. Our work forms part of an FAO/IAEA program on “Mutational analysis of root characters in annual food plants related to plant performance.” Work in Adelaide on AM colonization in tomato is supported by the Australian Research Council.

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Authors and Affiliations

  1. Estación Experimental del Zaidín (CSIC), calle Profesor Albareda 1, Granada, 18008, Spain

    Alberto Bago & Custodia Cano

  2. Soil and Land Systems, School of Earth and Environmental Sciences, Waite Campus, The University of Adelaide, Adelaide, 5005, Australia

    Jean-Patrick Toussaint, Sally Smith & Sandy Dickson

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  1. Alberto Bago
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  2. Custodia Cano
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  3. Jean-Patrick Toussaint
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  4. Sally Smith
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Correspondence to Alberto Bago.

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Bago, A., Cano, C., Toussaint, JP. et al. Interactions between the arbuscular mycorrhizal (AM) fungus Glomus intraradices and nontransformed tomato roots of either wild-type or AM-defective phenotypes in monoxenic cultures. Mycorrhiza 16, 429–436 (2006). https://doi.org/10.1007/s00572-006-0054-9

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