Abstract
Understanding the interactive effects and relationships between biochemical elements of tea leaves and the related factors, particularly climatic, cultivar, and geographic, is key for high-quality Ceylon tea production. The objectives of this study were to (1) investigate the effects of season × cultivar × agro-ecological regions (AERs) on the four tested biochemicals in fresh tea leaves, total polyphenol content (TPC), free sugar, protein, and theanine; (2) determine the relationships between, and develop a model to estimate, the biochemicals and their related factors; and (3) project the potential concentrations and distributions of four tested biochemicals in tea leaves with respect to the current and future climate. This study primarily uses inferential statistics via the Statistical Package for the Social Sciences (SPSS), cross-validation using R software, and the inverse distance weighting (IDW) approach in ArcGIS. The results demonstrate that the season, cultivar (Ceylon tea cultivars of TRI 2025 and TRI 4053), and AER and their interactions on biochemicals have significant effects (p < 0.05). The models derived in the regression analysis demonstrate the strong relationships between the independent variables and the biochemicals, with multiple correlation coefficients (R) around 0.8 and coefficient of determination (R2) around 0.6. The low standard deviation of error of prediction (SDEP < 0.1) and the high correlation coefficient of leave-one-out cross-validation (Q2) for all four biochemicals ranged from 0.56 to 0.61, which signifies the predictive ability of the models. The future projections show a considerable increase in the thresholds of all tested biochemicals. The distribution category with ‘very high’ concentrations of TPC and theanine is predicted to increase in the future by averages of 10% and 14%, respectively, while reducing the classes of protein and free sugar by 14% and 12%, respectively. Overall, the changing concentrations of the thresholds of relevant biochemicals and their distribution will negatively affect the final quality of tea, and these variations indicate that climate change has started to diminish Ceylon tea quality.
Similar content being viewed by others
References
Abeywickrama K, Ratnasooriya W, Amarakoon A (2010) Oral diuretic activity of hot water infusion of Sri Lankan black tea (Camellia sinensis L.) in rats. Pharmacogn Mag 6(24):271
Ahmad T, Akhlas M, Siyar HF (2003) Effect of seasonal variation and various nitrogen concentrations on polyphenols used as quality indicator of tea plant. Islam J Sci 13:1–8
Ahmed, S., 2014. Tea and the taste of climate change. Understanding impacts of environmental variation on botanical quality. Available online: https://sites. tufts. edu/teaandclimatechange/files/2014/07/Tea-andthe-Taste-of-Climate-Change. pdf (accessed on 7 June 2017)
Ahmed S, Orians CM, Griffin TS, Buckley S, Unachukwu U, Stratton AE, Stepp JR, Robbat A Jr, Cash S, Kennelly EJ (2014a) Effects of water availability and pest pressures on tea (Camellia sinensis) growth and functional quality. AoB Plants 6
Ahmed S, Stepp JR, Orians C, Griffin T, Matyas C, Robbat A, Cash S, Xue D, Long C, Unachukwu U, Buckley S, Small D, Kennelly E (2014b) Effects of extreme climate events on tea (Camellia sinensis) functional quality validate indigenous farmer knowledge and sensory preferences in tropical China. PLoS One 9(10):e109126
Ashihara H (2015) Occurrence, biosynthesis and metabolism of theanine (γ-glutamyl-L-ethylamide) in plants: a comprehensive review. Nat Prod Commun 10(5):1934578X1501000525
Banerji, S. and Willoughby, R., 2019. Addressing the human cost of Assam tea: an agenda for change to respect, protect and fulfil human rights on Assam tea plantations
Bassani DC, Nunes DS, Granato D (2014) Case study: quality control of Camellia sinensis and Ilex paraguariensis teas marketed in Brazil based on total phenolics, flavonoids and free-radical scavenging activity using chemometrics. Math Stat Methods Sci Technol:219–230
Bhagat R et al (2016) Report of the working group on climate change of the FAO intergovernmental group on tea. Food and Agriculture Organization of the United Nations, Rome
Bhuyan LP, Borah P, Sabhapondit S, Gogoi R, Bhattacharyya P (2015) Spatial variability of theaflavins and thearubigins fractions and their impact on black tea quality. J Food Sci Technol 52(12):7984–7993
Bian M, Skidmore AK, Schlerf M, Wang T, Liu Y, Zeng R, Fei T (2013) Predicting foliar biochemistry of tea (Camellia sinensis) using reflectance spectra measured at powder, leaf and canopy levels. ISPRS J Photogramm Remote Sens 78:148–156
Boehm R, Cash S, Anderson B, Ahmed S, Griffin T, Robbat A, Stepp J, Han W, Hazel M, Orians C (2016) Association between empirically estimated monsoon dynamics and other weather factors and historical tea yields in China: results from a yield response model. Climate 4(2):20
Boukarta, S. and Berezowska-Azzag, E., 2020. Exploring the role of socio-economic and built environment driving factors in shaping the commuting modal share: a path-analysis-based approach. Quaestiones Geographicae, 1(ahead-of-print)
Chen YL, Duan J, Jiang YM, Shi J, Peng L, Xue S, Kakuda Y (2010) Production, quality, and biological effects of oolong tea (Camellia sinensis). Food Rev Int 27(1):1–15
Chen L et al (2017) Therapeutic properties of green tea against environmental insults. J Nutr Biochem 40:13
Cheruiyot, E.K., Mumera, L.M., NG’ETICH, W.K., Hassanali, A. and Wachira, F., 2007. Polyphenols as potential indicators for drought tolerance in tea (Camellia sinensis L.). Bioscience, biotechnology, and biochemistry: 0707310505-0707310505
Chhogyel N, Kumar L, Bajgai Y, Jayasinghe LS (2020) Prediction of Bhutan's ecological distribution of rice (Oryza sativa L.) under the impact of climate change through maximum entropy modelling. J Agric Sci:1–13
Das A, Mukhopadhyay M, Sarkar B, Saha D, Mondal TK (2015) Influence of drought stress on cellular ultrastructure and antioxidant system in tea cultivars with different drought sensitivities. J Environ Biol 36(4):875–882
Data, W.-G.C., 2016. Free climate data for ecological modeling and GIS. Recuperado de: http://www. worldclim. org
De Costa W, Mohotti AJ, Wijeratne MA (2007) Ecophysiology of tea. Braz J Plant Physiol 19(4):299–332
Deng WW, Fei Y, Wang S, Wan XC, Zhang ZZ, Hu XY (2013) Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings. Plant Growth Regul 71(3):295–299
Diby L, Kahia J, Kouamé C, Aynekulu E (2017) Tea, coffee, and cocoa. Encycl. Appl. Plant Sci 3:420–425
Fischer G, Shah MN, Tubiello F, Van Velhuizen H (2005) Socio-economic and climate change impacts on agriculture: an integrated assessment, 1990–2080. Philos Trans Royal Soc B: Biol Sci 360(1463):2067–2083
Folin O, Ciocalteu V (1927) On tyrosine and tryptophane determinations in proteins. J Biol Chem 73(2):627–650
Ghabru A, Sud R (2017) Qualitative and quantitative evaluation of flavanols in Green tea [Camellia sinensis (L) O Kuntze]. Pharma Innovation 6(9, Part F):404
Golbraikh A, Tropsha A (2002) Beware of q2! J Mol Graph Model 20(4):269–276
Gramatica P (2014) External evaluation of QSAR models, in addition to cross-validation: verification of predictive capability on totally new chemicals. Molecular informatics 33(4):311–314
Gu L, Lu J, Ye B (2002) Tea chemistry. Chinese University of science and technology Publishing, Hefei
Gunathilaka RD, Smart JC, Fleming CM (2017) The impact of changing climate on perennial crops: the case of tea production in Sri Lanka. Clim Chang 140(3-4):577–592
Han W-Y, Huang JG, Li X, Li ZX, Ahammed GJ, Yan P, Stepp JR (2017) Altitudinal effects on the quality of green tea in east China: a climate change perspective. Eur Food Res Technol 243(2):323–330
Hazarika AK, Chanda S, Sabhapondit S, Sanyal S, Tamuly P, Tasrin S, Sing D, Tudu B, Bandyopadhyay R (2018) Quality assessment of fresh tea leaves by estimating total polyphenols using near infrared spectroscopy. J Food Sci Technol 55(12):4867–4876
Hilton P, Palmer-Jones R, Ellis R (1973) Effects of season and nitrogen fertiliser upon the flavanol composition and tea making quality of fresh shoots of tea (Camellia sinensis L.) in central Africa. J Sci Food Agric 24(7):819–826
Jayasekera S, Molan A, Garg M, Moughan P (2011) Variation in antioxidant potential and total polyphenol content of fresh and fully-fermented Sri Lankan tea. Food Chem 125(2):536–541
Jayasekera S, Kaur L, Molan A-L, Garg ML, Moughan PJ (2014) Effects of season and plantation on phenolic content of unfermented and fermented Sri Lankan tea. Food Chem 152:546–551
Jayasinghe SL, Kumar L (2019) Modeling the climate suitability of tea [Camellia sinensis (L.) O. Kuntze] in Sri Lanka in response to current and future climate change scenarios. Agric For Meteorol 272:102–117
Jayasinghe H, Suriyagoda L, Karunarathne A, Wijeratna M (2018) Modelling shoot growth and yield of Ceylon tea cultivar TRI-2025 (Camellia sinensis (L.) O. Kuntze). J Agric Sci 156(2):200–214
Jayasinghe S, Kumar L, Sandamali J (2019) Assessment of potential land suitability for tea (Camellia sinensis (L.) O. Kuntze) in Sri Lanka using a GIS-based multi-criteria approach. Agriculture 9(7):148
Jayasinghe SL, Kumar L, Hasan MK (2020) Relationship between environmental covariates and Ceylon tea cultivation in Sri Lanka. Agronomy 10(4):476
Juneja LR, Kapoor MP, Okubo T, Rao T (2013) Green tea polyphenols: nutraceuticals of modern life. CRC press
Kodama DH, Gonçalves AEdSS, Lajolo FM, Genovese MI (2010) Flavonoids, total phenolics and antioxidant capacity: comparison between commercial green tea preparations. Food Sci Tec 30(4):1077–1082
Kottawa-Arachchi, J., Gunasekare, M., Ranatunga, M., Jayasinghe, L. and Karunagoda, R., 2011. Analysis of selected biochemical constituents in black tea (Camellia sinensis) for predicting the quality of tea germplasm in Sri Lanka
Kottawa-Arachchi, J. et al., 2014. Biochemical characteristics of tea (Camellia L. spp.) germplasm accessions in Sri Lanka: correlation between black tea quality parameters and organoleptic evaluation. IJTS.
Kumar, S., Yadav, A., Yadav, M. and Yadav, J.P., 2017. Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm. f. BMC research notes, 10(1): 60.
Kwach BO, Owuor PO, Kamau DM, Wanyoko JK, Kamunya SM (2013) Influence of location of production, season and genotype on caffeine and flavan-3-ols in young green tea (Camellia sinensis) leaves in Kenya. J Agric Sci Technol B 3(8B):557
Lee J-E, Lee BJ, Chung JO, Hwang JA, Lee SJ, Lee CH, Hong YS (2010) Geographical and climatic dependencies of green tea (Camellia sinensis) metabolites: a 1H NMR-based metabolomics study. J Agric Food Chem 58(19):10582–10589
Lee J-E, Lee BJ, Chung JO, Kim HN, Kim EH, Jung S, Lee H, Lee SJ, Hong YS (2015) Metabolomic unveiling of a diverse range of green tea (Camellia sinensis) metabolites dependent on geography. Food Chem 174:452–459
Little MA et al (2017) Using and understanding cross-validation strategies. Perspectives on Saeb et al. GigaScience 6(5):gix020
Liu W, Su J, Li S, Lang X, Huang X (2018) Non-structural carbohydrates regulated by season and species in the subtropical monsoon broad-leaved evergreen forest of Yunnan Province, China. Sci Rep 8(1):1083
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lu GY, Wong DW (2008) An adaptive inverse-distance weighting spatial interpolation technique. Comput Geosci 34(9):1044–1055
Madanhire, J., 1995. Catechin variation in tea clones and the effect on liquor composition and quality, Proceedings of the First Regional Tea Seminar
Magoma G, Wachira F, Obanda M, Imbuga M, Agong S (2000) The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genet Resour Crop Evol 47(2):107–114
Mahanta PK (1988) Biochemical analysis as a measure of dynamic equilibrium in genomic setup during processing of tea. J Biosci 13(3):343–350
Menard S (2000) Coefficients of determination for multiple logistic regression analysis. Am Stat 54(1):17–24
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428
Mishra V, Kumar D, Ganguly AR, Sanjay J, Mujumdar M, Krishnan R, Shah RD (2014) Reliability of regional and global climate models to simulate precipitation extremes over India. J Geophys Res-Atmos 119(15):9301–9323
Muoki RC, Paul A, Kumar S (2012) A shared response of thaumatin like protein, chitinase, and late embryogenesis abundant protein3 to environmental stresses in tea [Camellia sinensis (L.) O. Kuntze]. Funct integr genom 12(3):565–571
Ng'etich W, Stephens W (2001) Responses of tea to environment in Kenya. 1. Genotype× environment interactions for total dry matter production and yield. Exp Agric 37(3):333–342
O’Donnell MS, Ignizio DA (2012) Bioclimatic predictors for supporting ecological applications in the conterminous United States. US Geol Surv Data Ser 691(10)
Obanda M, Owuor PO, Taylor SJ (1997) Flavanol composition and caffeine content of green leaf as quality potential indicators of Kenyan black teas. J Sci Food Agric 74(2):209–215
Obanda M, Owuor PO, Mang'oka R (2001) Changes in the chemical and sensory quality parameters of black tea due to variations of fermentation time and temperature. Food Chem 75(4):395–404
Owuor PO, Obaga SO, Othieno CO (1990) The effects of altitude on the chemical composition of black tea. J Sci Food Agric 50(1):9–17
Owuor PO, Obanda M, Nyirenda HE, Mandala WL (2008) Influence of region of production on clonal black tea chemical characteristics. Food Chem 108(1):263–271
Owuor PO et al (2011) Effects of genotype, environment and management on yields and quality of black tea, Genetics, Biofuels and Local Farming Systems. Springer, pp:277–307
Panabokke, C.R., 1996. Soils and agro-ecological environments of Sri Lanka. NARESA.
Panda H (2011) The complete book on cultivation and manufacture of tea. Asia Pacific Business Press Incorporated
Paul SK (2008) Predictability of price of tea from sensory assessments and biochemical information using data-mining techniques. J Sci Food Agric 88(8):1354–1362
Pérez-Burillo S, Giménez R, Rufián-Henares J, Pastoriza S (2018) Effect of brewing time and temperature on antioxidant capacity and phenols of white tea: relationship with sensory properties. Food Chem 248:111–118
Ranatunga MAB, Arachchi JDK, Gunasekare K, Yakandawala D (2017) Floral diversity and genetic structure of tea germplasm of Sri Lanka. Int J Biodivers:2017
Rigden A, Ongoma V, Huybers P (2020) Kenyan tea is made with heat and water: how will climate change influence its yield? Environ Res Lett 15(4):044003
Roberts E, Smith R (1963) The phenolic substances of manufactured tea. IX.—The spectrophotometric evaluation of tea liquors. J Sci Food Agric 14(10):689–700
Robertson A (1992) The chemistry and biochemistry of black tea production—the non-volatiles, Tea. Springer, pp:555–601
Samarasinghe, K., Jayasinghe, H. and Alakolanga, A., 2018. Evaluation of the relationship between SPAD reading and biochemical profile of fresh leaves of selected tea cultivars (Camellia sinensis (L.) O. Kuntze). 2nd international research symposium, I(Uva Wellassa University of Sri Lanka): 524
Sanderson GW (1972) The chemistry of tea and tea manufacturing, Recent advances in phytochemistry. Elsevier, pp:247–316
Sanderson, G. and Perera, B., 1966. Carbohydrates in tea plants-2-the carbohydrates in tea roots.
Saravanan M, John KM, Kumar RR, Pius P, Sasikumar R (2005) Genetic diversity of UPASI tea clones (Camellia sinensis (L.) O. Kuntze) on the basis of total catechins and their fractions. Phytochemistry 66(5):561–565
Saunders LJ, Russell RA, Crabb DP (2012) The coefficient of determination: what determines a useful R2 statistic? Invest Ophthalmol Vis Sci 53(11):6830–6832
Schmidheiny K (2013) The multiple linear regression model. Short Guides to Microeconometrics, Version 20:29
Schneider F, Maurer C, Friedberg RC (2017) International organization for standardization (ISO) 15189. Annals of laboratory medicine 37(5):365–370
Seo S-nN, Mendelsohn R, Munasinghe M (2005) Climate change and agriculture in Sri Lanka: a Ricardian valuation. Environ Dev Econ 10(5):581–596
Sharmila S, Joseph S, Chattopadhyay R, Sahai A, Goswami B (2015) Asymmetry in space–time characteristics of Indian summer monsoon intraseasonal oscillations during extreme years: Role of seasonal mean state. Int J Climatol 35(8):1948–1963
Soni RP, Katoch M, Kumar A, Ladohiya R, Verma P (2015) Tea: production, composition, consumption and its potential as an antioxidant and antimicrobial agent. Int J Food Ferment Technol 5(2):95–106
SPSS., 2010. SPSS version 6.1 computer program, version 19.0. SPSS Chicago, IL.
Teshome K (2019) Effect of tea processing methods on biochemical composition and sensory quality of black tea (Camellia sinensis (L.) O. Kuntze): a review. J Hortic For 11(6):84–95
TRI (2001) Climate change impact on tea. Tea Research Institute, Talawakelle
Uchiyama S, Taniguchi Y, Saka A, Yoshida A, Yajima H (2011) Prevention of diet-induced obesity by dietary black tea polyphenols extract in vitro and in vivo. Nutrition 27(3):287–292
Voora, V., Bermúdez, S. and Larrea, C., 2019. Global market report: tea, International Institute for Sustainable Development (IISD), Canada.
Wei K, Wang LY, Zhou J, He W, Zeng JM, Jiang YW, Cheng H (2012) Comparison of catechins and purine alkaloids in albino and normal green tea cultivars (Camellia sinensis L.) by HPLC. Food Chem 130(3):720–724
Wijeratne, M., 2004. Effect of soil moisture deficit and temperature on dry matter accumulation in tea shoots.
Wijeratne M, Lalith C (2013) Analysis of rainfall and temperature in tea growing agro-ecological regions and assessment of vulnerability of tea plantations in Sri Lanka to climate change. Sri Lanka J Tea Sci 78(1/2):42–59
Williams MN, Grajales CAG, Kurkiewicz D (2013) Assumptions of multiple regression: correcting two misconceptions. Pract Assess Res Eval 18(1):11
Williams J, Sergi D, McKune AJ, Georgousopoulou EN, Mellor DD, Naumovski N (2019) The beneficial health effects of green tea amino acid l-theanine in animal models: promises and prospects for human trials. Phytother Res 33(3):571–583
Wright L (2005) Biochemical analyses for identification of quality in black tea (Camellia sinensis): submitted… for the degree Ph. D. Bioch, Pretoria
Xiao J, Huo J, Jiang H, Yang F (2011) Chemical compositions and bioactivities of crude polysaccharides from tea leaves beyond their useful date. Int J Biol Macromol 49(5):1143–1151
Yang L et al (2018) Response of plant secondary metabolites to environmental factors. Molecules 23(4):762
Yao L, Caffin N, D'arcy B, Jiang Y, Shi J, Singanusong R, Liu X, Datta N, Kakuda Y, Xu Y (2005) Seasonal variations of phenolic compounds in Australia-grown tea (Camellia sinensis). J Agric Food Chem 53(16):6477–6483
Yashin AY, Nemzer BV, Combet E, Yashin YI (2015) Determination of the chemical composition of tea by chromatographic methods: a review. J Food Res 4(3):56–87
Ying Y, Ho JW, Chen ZY, Wang J (2005) Analysis of theanine in tea leaves by HPLC with fluorescence detection. J Liq Chromatogr Relat Technol 28(5):727–737
Zhang Y, Chen H, Zhang N, Ma L (2015) Antioxidant and functional properties of tea protein as affected by the different tea processing methods. J Food Sci Technol 52(2):742–752
Acknowledgements
This research was supported by a postgraduate scholarship provided by the University of New England, Australia, to the first author. The authors wish to thank the tea plantation companies in Sri Lanka for permitting to obtain leaf samples. The authors extend their thanks to Mrs. Achala Alakolanga, Mrs. Jeewanthi Polegoda, Ms. Bhaggya Samarasinghe, Mr. Imalka Samarakoon, Mr. Sandun Nirmala, Mr. T. Thiruneelan, and Ms. Ishanka De Silva for assisting in collecting and analysing chemicals of tea leaf samples. The authors also acknowledge Dr. Harsha Kadupitiya, Additional Director, Natural Resources Management Center, Peradeniya, Sri Lanka, who provided data on agro-ecological regions of Sri Lanka.
Author information
Authors and Affiliations
Corresponding author
Supplementary Information
ESM 1
(DOCX 1423 kb)
Rights and permissions
About this article
Cite this article
Jayasinghe, S.L., Kumar, L. & Kaliyadasa, E. The future of high-quality Ceylon tea seems bleak in the face of climate change. Int J Biometeorol 65, 1629–1646 (2021). https://doi.org/10.1007/s00484-021-02118-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00484-021-02118-9