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The impact of dialysis solution biocompatibility on ultrafiltration and on free water transport in rats

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Abstract

This study compares different peritoneal dialysis fluids (PDF) in rats over a short contact time. For greater accuracy, net ultrafiltration (UF) and peritoneal transport indices, mass transfer area coefficient (MTAC) were scaled for the in vivo peritoneal surface area recruited (ivPSA) measured by microcomputerized tomography. Wistar rats underwent nephrectomy (5/6ths), were randomized into two groups and given 1.5% glucose PDF, either conventional acidic lactate (n = 14) or pH neutral bicarbonate (BicaVera) (n = 13); MTAC and UF were measured using a 90-min peritoneal equilibrium test (PET), fill volume (IPV) of 10 ml/100 g; small pore fluid transport was determined from sodium balance and used to calculate free water transport (FWT). Each ivPSA value was significantly correlated with the actual IPV, which varied from one rat to another. At 90 min of contact, there was no difference in recruited ivPSA in relation to PDFs. There was a difference (p < 0.01) in net UF/ivPSA 0.45 vs. 1.41 cm2/ml for bicarbonate versus lactate, as there was in the proportion of FWT with bicarbonate (42 ± 5% of net UF) compared to lactate (29 ± 4% of net UF). Net UF for individual values of ivPSA differs between conventional PDF and more biocompatible solutions, such as bicarbonate PDF. This observed change in UF cannot be fully explained by differences in glucose transport. The changes in FWT may be explained by the impact of the PDF biocompatibility on aquaporin function.

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Acknowledgments

This research was supported in part by grants from Fresenius Medical Care, Dr. Redouanne Taamma. The authors have no conflicts of interest to declare. The preparation of this review was not supported by any external funding. During the peer-review process, the manufacturer of the agent under review was offered an opportunity to comment on this article. Changes resulting from comments received were made on the basis of scientific and editorial merit.

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Authors and Affiliations

  1. Service de biophysique et médecine nucléaire – Institut de Mécanique des fluides et des Solides CNRS Université de Strasbourg, CHU Hautepierre, Avenue Molière, 67098, Strasbourg Cedex, France

    Gaëlle Aubertin, Philippe Choquet & André Constantinesco

  2. Service de Pédiatrie 1, CHU Hautepierre, Avenue Molière, 67098, Strasbourg Cedex, France

    Céline Dheu, Ariane Zaloszyc & Michel Fischbach

  3. Laboratoire de Biochimie et Biologie Moléculaire, CHU Hautepierre, Avenue Molière, 67098, Strasbourg Cedex, France

    Charline Ratomponirina

  4. Department of Nephrology, University Hospital Düsseldorf, Moorenstrasse 5, 40225, Duesseldorf, Germany

    Jutta Passlick-Deetjen

  5. Nephrology Dialysis Transplantation Children’s Unit, University Hospital Hautepierre, Avenue Molière, 67098, Strasbourg, France

    Michel Fischbach

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  1. Gaëlle Aubertin
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  2. Philippe Choquet
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  3. Céline Dheu
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  4. André Constantinesco
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  5. Charline Ratomponirina
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  6. Ariane Zaloszyc
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  7. Jutta Passlick-Deetjen
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  8. Michel Fischbach
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Correspondence to Michel Fischbach.

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Aubertin, G., Choquet, P., Dheu, C. et al. The impact of dialysis solution biocompatibility on ultrafiltration and on free water transport in rats. Pediatr Nephrol 27, 131–138 (2012). https://doi.org/10.1007/s00467-011-1945-3

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  • DOI: https://doi.org/10.1007/s00467-011-1945-3

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