Abstract
Several genome-wide association studies (GWASs) have reported associations between single nucleotide polymorphisms (SNPs) and uric acid concentrations or gout in a number of different ethnic populations. To clarify the global relevance of the previously identified SNPs in the development of the qualitative trait gout, in the present study, the associations between two SNPs in the glucokinase (hexokinase 4) regulator (GCKR) gene and gout were assessed in a male Chinese Han population. The study population comprised 476 male gout patients and 465 male controls. Multiple PCR was performed using time-of-flight mass spectrometry (MALDI-TOF MS) to identify genotypes. Two SNPs, rs780093 and rs780094, located in intronic regions of the GCKR gene were found to be significantly associated with the development of gout. Thus, the association between the two GCKR SNPs and gout was replicated in the male Han Chinese population investigated in the present study. Furthermore, GCKR was identified as a novel candidate gene associated with gout.
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Acknowledgments
This work was supported by the National Infrastructure Program of Chinese Genetic Resources (2006DKA21300), the National Basic Research Program of China (2010CB534902), the National Science & Technology Pillar Program of China (No.2008BAH24B05), and the National Natural Science and Foundation of China (30871192).
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The authors J. Wang and S. Liu contributed equally to the work.
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Wang, J., Liu, S., Wang, B. et al. Association between gout and polymorphisms in GCKR in male Han Chinese. Hum Genet 131, 1261–1265 (2012). https://doi.org/10.1007/s00439-012-1151-9
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DOI: https://doi.org/10.1007/s00439-012-1151-9