Abstract
Purpose
The clinical importance of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC) is well recognized. However, a reliable method for the detection of functioning CSC has not yet been established. We hypothesized that YAP1, a transcriptional coactivator, and SOX2, a master transcription factor of SCC, may cooperatively induce stemness through transcriptional reprogramming.
Methods
We immunohistochemically examined the expression of SOX2 and YAP1 in the CD44 variant 9 (CD44v9)-positive invasion front. A CSC-inducible module was identified through a combination of siRNAs and sphere formation assays. YAP1 and SOX2 interactions were analyzed in vitro.
Results
The triple overexpression of SOX2, YAP1, and CD44v9 was significantly associated with poor prognosis. TCGA data revealed that the CSC-inducible module, which was related to EMT and angiogenesis, was significantly correlated with poor prognosis. The KLF7 expression, representatively chosen from the module, also correlated with poor prognosis and was essential for sphere formation and CSC propagation. Sphere stress-activated YAP1 enhanced SOX2 activity.
Conclusions
The stress-triggered activation of YAP1/SOX2 transcriptionally reprograms HNSCC for the acquisition of stemness. Triple SOX2, YAP1, and CD44v9 immunostaining assays may be useful for the selection of high-risk patients with functioning CSCs, and YAP1 targeting may lead to the development of a CSC-targeting therapy.
Similar content being viewed by others
Abbreviations
- YAP1:
-
Yes-associated protein 1
- SOX2:
-
SRY (sex determining region Y)-box 2
- KLF:
-
Krüppel-like family of transcription factor
- OCT:
-
Octamer-binding Transcription Factor
- MMP:
-
Matrix metalloproteinase
- SLCO2A1:
-
Solute carrier organic anion transporter family, member 2A1
- SERPINB2:
-
Serpin family B member 2
- LEMD1:
-
LEM domain-containing 1
- DDX60:
-
DExD/H-box helicase 60
- BRD4:
-
Bromodomain containing 4
- TEAD:
-
TEA domain transcription factor
- FOXM1:
-
Forkhead box M1
- CYR61:
-
Cysteine-rich angiogenic inducer 61
- CTGF:
-
Connective Tissue Growth Factor
References
Aso T et al (2015) Induction of CD44 variant 9-expressing cancer stem cells might attenuate the efficacy of chemoradioselection and Worsens the prognosis of patients with advanced head and neck cancer. PLoS One 10:e0116596. https://doi.org/10.1371/journal.pone.0116596
Bahmad HF et al (2018) Sphere-formation assay: three-dimensional in vitro culturing of prostate cancer stem/progenitor sphere-forming cells. Front Oncol 8:347. https://doi.org/10.3389/fonc.2018.00347
Basu-Roy U, Bayin NS, Rattanakorn K, Han E, Placantonakis DG, Mansukhani A, Basilico C (2015) Sox2 antagonizes the Hippo pathway to maintain stemness in cancer cells. Nat Commun 6:6411. https://doi.org/10.1038/ncomms7411
Baumeister P et al (2018) High expression of EpCAM and Sox2 is a positive prognosticator of clinical outcome for head and neck carcinoma. Sci Rep 8:14582. https://doi.org/10.1038/s41598-018-32178-8
Bora-Singhal N, Nguyen J, Schaal C, Perumal D, Singh S, Coppola D, Chellappan S (2015) YAP1 regulates OCT4 activity and SOX2 Expression to facilitate self-renewal and vascular mimicry of stem-like cells. Stem Cells 33:1705–1718. https://doi.org/10.1002/stem.1993
Boumahdi S et al (2014) SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma. Nature 511:246–250. https://doi.org/10.1038/nature13305
Bradner JE, Hnisz D, Young RA (2017) Transcriptional addiction in cancer. Cell 168:629–643. https://doi.org/10.1016/j.cell.2016.12.013
Cancer Genome Atlas Network (2015) Comprehensive genomic characterization of head and neck squamous cell carcinomas. Nature 517:576–582. https://doi.org/10.1038/nature14129
da Huang W, Sherman BT, Lempicki RA (2009a) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res 37:1–13. https://doi.org/10.1093/nar/gkn923
da Huang W, Sherman BT, Lempicki RA (2009b) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 4:44–57. https://doi.org/10.1038/nprot.2008.211
Di Agostino S et al (2016) YAP enhances the pro-proliferative transcriptional activity of mutant p53 proteins. EMBO Rep 17:188–201. https://doi.org/10.15252/embr.201540488
Ding X, Wang X, Gong Y, Ruan H, Sun Y, Yu Y (2017) KLF7 overexpression in human oral squamous cell carcinoma promotes migration and epithelial–mesenchymal transition. Oncol Lett 13:2281–2289. https://doi.org/10.3892/ol.2017.5734
Dong Z, Liu G, Huang B, Sun J, Wu D (2014) Prognostic significance of SOX2 in head and neck cancer: a meta-analysis. Int J Clin Exp Med 7:5010–5020
Elbediwy A, Vincent-Mistiaen ZI, Thompson BJ (2016) YAP and TAZ in epithelial stem cells: a sensor for cell polarity, mechanical forces and tissue damage. BioEssays 38:644–653. https://doi.org/10.1002/bies.201600037
Eun YG et al (2017) Clinical significance of YAP1 activation in head and neck squamous cell carcinoma. Oncotarget 8:111130–111143. https://doi.org/10.18632/oncotarget.22666
Feinberg AP, Koldobskiy MA, Gondor A (2016) Epigenetic modulators, modifiers and mediators in cancer aetiology and progression. Nat Rev Genet 17:284–299. https://doi.org/10.1038/nrg.2016.13
Fu TY et al (2016) Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma. Oncotarget 7:85097–85108. https://doi.org/10.18632/oncotarget.13197
Garcia-Escudero R et al (2018) Overexpression of PIK3CA in head and neck squamous cell carcinoma is associated with poor outcome and activation of the YAP pathway. Oral Oncol 79:55–63. https://doi.org/10.1016/j.oraloncology.2018.02.014
Ge L et al (2011) Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis. PLoS One 6:e27529. https://doi.org/10.1371/journal.pone.0027529
Giesen C et al (2014) Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nat Methods 11:417–422. https://doi.org/10.1038/nmeth.2869
Harris NLE et al (2017) SerpinB2 regulates stromal remodelling and local invasion in pancreatic cancer. Oncogene 36:4288–4298. https://doi.org/10.1038/onc.2017.63
Hiemer SE et al (2015) A YAP/TAZ-regulated molecular signature is associated with oral squamous cell carcinoma. Mol Cancer Res 13:957–968. https://doi.org/10.1158/1541-7786.MCR-14-0580
Hirata H et al (2016) Decreased expression of fructose-1,6-bisphosphatase associates with glucose metabolism and tumor progression in hepatocellular carcinoma. Cancer Res 76:3265–3276. https://doi.org/10.1158/0008-5472.can-15-2601
Jiang Y et al (2018) Co-activation of super-enhancer-driven CCAT1 by TP63 and SOX2 promotes squamous cancer progression. Nat Commun 9:3619. https://doi.org/10.1038/s41467-018-06081-9
Jin T et al (2017) microRNA-200c/141 upregulates SerpinB2 to promote breast cancer cell metastasis and reduce patient survival. Oncotarget 8:32769–32782. https://doi.org/10.18632/oncotarget.15680
Keysar SB et al (2017) Regulation of head and neck squamous cancer stem cells by PI3K and SOX2J. Natl Cancer Inst 1:1. https://doi.org/10.1093/jnci/djw189
Lee SH et al (2014) SOX2 regulates self-renewal and tumorigenicity of stem-like cells of head and neck squamous cell carcinoma. Br J Cancer 111:2122–2130. https://doi.org/10.1038/bjc.2014.528
Leemans CR, Snijders PJF, Brakenhoff RH (2018) Publisher Correction: The molecular landscape of head and neck cancer. Nat Rev Cancer 18:662. https://doi.org/10.1038/s41568-018-0057-9
Lengerke C et al (2011) Expression of the embryonic stem cell marker SOX2 in early-stage breast carcinoma. BMC Cancer 11:42. https://doi.org/10.1186/1471-2407-11-42
Lian I et al (2010) The role of YAP transcription coactivator in regulating stem cell self-renewal and differentiation. Genes Dev 24:1106–1118. https://doi.org/10.1101/gad.1903310
Limame R, Op de Beeck K, Lardon F, De Wever O, Pauwels P (2014) Kruppel-like factors in cancer progression: three fingers on the steering wheel. Oncotarget 5:29–48. https://doi.org/10.18632/oncotarget.1456
Masuda M, Toh S, Wakasaki T, Suzui M, Joe AK (2013) Somatic evolution of head and neck cancer—biological robustness and latent vulnerability. Mol Oncol 7:14–28. https://doi.org/10.1016/j.molonc.2012.10.009
Masuda M, Wakasaki T, Toh S (2016) Stress-triggered atavistic reprogramming (STAR) addiction: driving force behind head and neck cancer? Am J Cancer Res 6:1149–1166
Mistri TK et al (2015) Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells. EMBO Rep 16:1177–1191. https://doi.org/10.15252/embr.201540467
Miyahara E, Nishikawa T, Takeuchi T, Yasuda K, Okamoto Y, Kawano Y, Horiuchi M (2014) Effect of myeloperoxidase inhibition on gene expression profiles in HL-60 cells exposed to 1,2,4,-benzenetriol. Toxicology 317:50–57. https://doi.org/10.1016/j.tox.2014.01.007
Morris LGT et al (2017) The molecular landscape of recurrent and metastatic head and neck cancers: insights from a precision oncology sequencing platform. JAMA Oncol 3:244–255. https://doi.org/10.1001/jamaoncol.2016.1790
Nakatani K et al (2017) Targeting the Hippo signalling pathway for cancer treatment. J Biochem 161:237–244. https://doi.org/10.1093/jb/mvw074
Nishio M et al (2016) Dysregulated YAP1/TAZ and TGF-beta signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci USA 113:E71–E80. https://doi.org/10.1073/pnas.1517188113
Ooki A et al (2018) YAP1 and COX2 coordinately regulate urothelial cancer stem-like cells. Cancer Res 78:168–181. https://doi.org/10.1158/0008-5472.can-17-0836
Saladi SV et al (2017) ACTL6A is co-amplified with p63 in squamous cell carcinoma to drive YAP activation, regenerative proliferation, and poor prognosis. Cancer Cell 31:35–49. https://doi.org/10.1016/j.ccell.2016.12.001
Sasahira T, Kurihara M, Nakashima C, Kirita T, Kuniyasu H (2016) LEM domain containing 1 promotes oral squamous cell carcinoma invasion and endothelial transmigration. Br J Cancer 115:52–58. https://doi.org/10.1038/bjc.2016.167
Segrelles C, Paramio JM, Lorz C (2018) The transcriptional co-activator YAP: a new player in head and neck cancer. Oral Oncol 86:25–32. https://doi.org/10.1016/j.oraloncology.2018.08.020
Seo E, Basu-Roy U, Gunaratne PH, Coarfa C, Lim DS, Basilico C, Mansukhani A (2013) SOX2 regulates YAP1 to maintain stemness and determine cell fate in the osteo-adipo lineage. Cell Rep 3:2075–2087. https://doi.org/10.1016/j.celrep.2013.05.029
Shibue T, Weinberg RA (2017) EMT, CSCs, and drug resistance: the mechanistic link and clinical implications. Nat Rev Clin Oncol 14:611–629. https://doi.org/10.1038/nrclinonc.2017.44
Subramanian A et al (2005) Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA 102:15545–15550. https://doi.org/10.1073/pnas.0506580102
Suva ML, Riggi N, Bernstein BE (2013) Epigenetic reprogramming in cancer. Science 339:1567–1570. https://doi.org/10.1126/science.1230184
Totaro A, Panciera T, Piccolo S (2018) YAP/TAZ upstream signals and downstream responses. Nat Cell Biol 20:888–899. https://doi.org/10.1038/s41556-018-0142-z
Tsujikawa T et al (2017) Quantitative multiplex immunohistochemistry reveals myeloid-inflamed tumor-immune complexity associated with poor prognosis. Cell Rep 19:203–217. https://doi.org/10.1016/j.celrep.2017.03.037
Watanabe H et al (2014) SOX2 and p63 colocalize at genetic loci in squamous cell carcinomas. J Clin Investig 124:1636–1645
Wei X, Ye J, Shang Y, Chen H, Liu S, Liu L, Wang R (2017) Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells. Oncotarget 8:109301–109318. https://doi.org/10.18632/oncotarget.22673
Weiler SME et al (2017) Induction of chromosome instability by activation of yes-associated protein and forkhead box M1 in liver. Cancer Gastroenterol 152:2037–2051, e2022. https://doi.org/10.1053/j.gastro.2017.02.018
Yoshikawa M et al (2013) xCT inhibition depletes CD44v-expressing tumor cells that are resistant to EGFR-targeted therapy in head and neck squamous cell carcinoma. Cancer Res 73:1855–1866. https://doi.org/10.1158/0008-5472.can-12-3609-t
Zanconato F, Cordenonsi M, Piccolo S (2016) YAP/TAZ at the roots of cancer. Cancer Cell 29:783–803. https://doi.org/10.1016/j.ccell.2016.05.005
Zanconato F et al (2018) Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4. Nat Med 24:1599–1610. https://doi.org/10.1038/s41591-018-0158-8
Zehir A et al (2017) Mutational landscape of metastatic cancer revealed from prospective clinical sequencing of 10,000 patients. Nat Med 23:703–713. https://doi.org/10.1038/nm.4333
Zhu Q, Liang X, Dai J, Guan X (2015) Prostaglandin transporter, SLCO2A1, mediates the invasion and apoptosis of lung cancer cells via PI3K/AKT/mTOR pathway. Int J Clin Exp Pathol 8:9175–9181
Acknowledgements
We would like to thank J. S. Gutkind (University of California) for providing the Cal33 cells and Ms. Kimiko Baba for her excellent technical support.
Funding
We are grateful for the funding provided by the Japanese Society for the Promotion of Science (MEXT/JSPS KAKENHI Grant Number JP16K11255 to M.M., and JP16K11256 to T.W.).
Author information
Authors and Affiliations
Contributions
Conceptualization: HO, KS, TW, ST, MM; analysis: HO, KS, TN, KT, MM; data curation: HO, KS, TN; writing of the original draft: HO, MM; supervision: TW, ST, TN; project administration: MM; funding acquisition: TW, MM.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Study approval
All clinical samples were approved for analysis by the Ethics Committee at National Hospital Organization Kyushu Cancer Center (2015-43).
Informed consent
Written informed consent was obtained from all individual participants included in this study.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Omori, H., Sato, K., Nakano, T. et al. Stress-triggered YAP1/SOX2 activation transcriptionally reprograms head and neck squamous cell carcinoma for the acquisition of stemness. J Cancer Res Clin Oncol 145, 2433–2444 (2019). https://doi.org/10.1007/s00432-019-02995-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00432-019-02995-z