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Identification of CTNNB1-PLAG1 gene rearrangement in a patient with pulmonary pleomorphic adenoma

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Abstract

Pulmonary pleomorphic adenoma (PA) is a rare salivary gland-type neoplasm, which predominantly occurs in the proximal airway. Rearrangement of the pleomorphic adenoma gene 1 (PLAG1) is the most frequent genetic event in PAs of salivary glands. However, whether pulmonary PA also harbors PLAG1 rearrangement has not been elucidated. Here, we present a case of pulmonary PA, located at the middle lobar bronchus, in a 54-year-old man. CTNNB1-PLAG1 gene fusion was identified by reverse transcription-polymerase chain reaction using formalin-fixed paraffin-embedded tissue (FFPE). Furthermore, immunohistochemical analysis revealed nuclear expression of PLAG1 in all tumor cells. To the best of our knowledge, this is the first reported case of pulmonary PA with CTNNB1-PLAG1 fusion and PLAG1 expression. Our case illustrates the possibility that pulmonary PA could be underpinned by recurrent PLAG1 translocations akin to salivary gland PA.

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Funding

This study was supported in part through grants by the Grant-in-Aid for Scientific Research (KAKENHI) program of Japan Society for the Promotion of Science (JSPS) (Grant Number #19K07469), and by the Takeda Science Foundation.

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Authors and Affiliations

  1. Department of Human Pathology, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan

    Miki Asahina, Takuo Hayashi, Tsuyoshi Saito, Hiroko Onagi, Monami Kishi, Yuki Fukumura, Atsushi Arakawa & Takashi Yao

  2. Department of General Thoracic Surgery, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan

    Kazuya Takamochi & Kenji Suzuki

Authors
  1. Miki Asahina
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  2. Takuo Hayashi
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  3. Kazuya Takamochi
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  4. Tsuyoshi Saito
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  5. Hiroko Onagi
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  6. Monami Kishi
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  7. Yuki Fukumura
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  8. Atsushi Arakawa
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  9. Kenji Suzuki
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  10. Takashi Yao
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Contributions

Miki Asahina and Takuo Hayashi conceived and designed the study, and wrote edited and reviewed the manuscript. Kazuya Takamochi and Kenji Suzuki provided and gave patient’s clinical information. Miki Asahina, Takuo Hayashi, Tsuyoshi Saito, Hiroko Onagi, Monami Kishi, Yuki Fukumura, Atsushi Arakawa, and Takashi Yao provided pathological information. All authors gave final approval for publication. Takuo Hayashi takes full responsibility for the work as a whole, including the study design, access to data, and the decision to submit and publish the manuscript.

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Correspondence to Takuo Hayashi.

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Asahina, M., Hayashi, T., Takamochi, K. et al. Identification of CTNNB1-PLAG1 gene rearrangement in a patient with pulmonary pleomorphic adenoma. Virchows Arch 477, 739–742 (2020). https://doi.org/10.1007/s00428-020-02810-y

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  • DOI: https://doi.org/10.1007/s00428-020-02810-y

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