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Cyclosporin A in the ocular fluids of uveitis patients following long-term systemic administration

  • Inflammatory Disorders
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Abstract

Background/aims

To determine the levels of cyclosporin A (CsA) in tears and the anterior segment of the eye following long-term oral intake for autoimmune diseases.

Methods

Subjects taking oral CsA to treat relapsing autoimmune ocular inflammation were included in this study. All of the patients had been quiescent for at least 6 months. In patients scheduled for cataract extraction (group A), the CsA levels in the blood, aqueous humour and anterior capsule of the lens were determined. In subjects not requiring surgical intervention (group B), CsA was measured in tears and blood. The samples were analysed using turbulent flow chromatography coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results

There were 19 subjects in group A and 43 subjects in group B. CsA was detectable in all of the tear samples with a mean value of 22.4 ± 20.2 ng/ml and there was a significant positive correlation between the CsA levels in tears and blood (P = 0.012). CsA was not detected in any of the surgical samples.

Conclusion

LC-MS/MS proved very sensitive for detecting CsA in low-volume biological samples. CsA was present in human tears in proportion to the blood level after an average of 12 hours from the last oral intake.

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Authors and Affiliations

  1. Institute of Ophthalmology, University of Parma, Via Gramsci 14, 43100, Parma, Italy

    Paolo Mora, Francesca Manzotti, Arturo Carta, Raffaella Aldigeri & Jelka G. Orsoni

  2. Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Leipzig, Germany

    Uta Ceglarek

  3. Paediatric Division, General Hospital of Parma, Parma, Italy

    Laura Zavota

Authors
  1. Paolo Mora
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  2. Uta Ceglarek
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  3. Francesca Manzotti
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  4. Laura Zavota
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  5. Arturo Carta
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  6. Raffaella Aldigeri
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  7. Jelka G. Orsoni
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Corresponding author

Correspondence to Paolo Mora.

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Financial relationship: none.

The authors have full control of all primary data and they agree to allow Graefes Archive for Clinical and Experimental Ophthalmology to review their data if requested.

The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, see: http://www.textcheck.com/cgi-bin/certificate.cgi?id=vYM0nn

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Mora, P., Ceglarek, U., Manzotti, F. et al. Cyclosporin A in the ocular fluids of uveitis patients following long-term systemic administration. Graefes Arch Clin Exp Ophthalmol 246, 1047–1052 (2008). https://doi.org/10.1007/s00417-008-0825-4

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  • DOI: https://doi.org/10.1007/s00417-008-0825-4

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