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Total IgM and Anti-Phosphatidylcholine IgM Antibody Secretion Continue After Clearance of Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection

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Abstract

The cellular immune response to Mycobacterium tuberculosis infection has been well characterized, while the humoral antibody response remains underexplored. We aimed to examine the total and anti-phospholipid IgM levels in the pleural lavage from mice with Mycobacterium bovis BCG extrapulmonary infection. We found that the levels of total and anti-phosphatidylcholine IgM antibodies remained significantly higher in infected mice as compared to non-infected mice up to day 90 after BCG infection, while the anti-cardiolipin IgM antibody levels decreased with bacteria clearance. Our findings suggest that IgM antibodies are secreted and their composition vary during early and late immune response to BCG pleurisy.

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Acknowledgements

We thank Fermín Acosta for conducting the morphologic analysis and DNA identification of the Mycobacterium bovis BCG culture. We thank Yisett González for her assistance with the animal experiments at INDICASAT-AIP and specially thank Colleen Goodridge for her critical review of the manuscript and valuable suggestions.

Funding

This study was funded by a Doctoral Fellowship from the Secretaria Nacional de Ciencia Tecnología e Innovación (SENACYT) and the Instituto para la Formación y Aprovechamiento de los Recursos Humanos (IFARHU) (Grant No. IFARHU-270-2012-138). We also received funds from SENACYT (Grant Nos. ITE-11-020, GC-2015-22), as well as from the Sistema Nacional de Investigadores de Panamá (SNI) (Grants Nos. SNI-55-2014, SNI-129-2015).

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Authors and Affiliations

  1. Tuberculosis Biomarker Research Unit, Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), City of Knowledge, Panama, Panama

    Ciara Ordoñez, Musharaf Tarajia, Dilcia Sambrano, Victoria Batista & Amador Goodridge

  2. Department of Biotechnology, Acharya Nargajuna University, Guntur, India

    Ciara Ordoñez & Musharaf Tarajia

  3. Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), City of Knowledge, Panama, Panama

    René Rivera & Patricia L. Fernández

  4. Facultad de Medicina, Universidad de Panamá, Panama, Panama

    Mónica Chávez & Denis Tapia

Authors
  1. Ciara Ordoñez
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  2. Musharaf Tarajia
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  3. René Rivera
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  4. Dilcia Sambrano
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  5. Victoria Batista
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  6. Mónica Chávez
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  7. Denis Tapia
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  8. Patricia L. Fernández
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  9. Amador Goodridge
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Corresponding author

Correspondence to Amador Goodridge.

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All authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Ethical approval was obtained from Institutional Animal Care and Use Committee of INDICASAT-AIP. This article does not contain any studies with human participants performed by any of the authors.

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Ordoñez, C., Tarajia, M., Rivera, R. et al. Total IgM and Anti-Phosphatidylcholine IgM Antibody Secretion Continue After Clearance of Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection. Lung 195, 517–521 (2017). https://doi.org/10.1007/s00408-017-0019-0

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  • DOI: https://doi.org/10.1007/s00408-017-0019-0

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