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Activations of group 2 innate lymphoid cells depend on endotypes of chronic rhinosinusitis

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Objective

Chronic rhinosinusitis (CRS) is a complicated disease with several variants caused by different cellular and molecular mechanisms. The characterization of this heterogeneity supports the definition that the disease consists of many endotypes, such as eosinophilic and neutrophilic CRS, and so on. This study aimed to explore group 2 innate lymphoid cells (ILC2s) in neutrophilic CRS without nasal polyps (CRSsNP) and with nasal polyps (CRSwNP), and evaluate ILC2s across characteristics of the disease.

Methods

Nasal biopsy samples were obtained from normal subjects or subjects with CRSsNP or CRSwNP during surgery. ILC2s were sorted and purified as CD45+Lin−CD127+CD4−CD8−CRTH2+CD161+ cells through flow cytometry, and were compared among three groups of subjects. Then, these samples were cultured in vitro, and inflammatory factors were assessed in tissue cultures. After that, human recombinant (rm) interleukin (IL)-33 or IL-17 were administered into the cultures, and we again examined relevant inflammatory substances.

Results

ILC2s were upregulated in neutrophilic CRSsNP and CRSwNP patients, and there were no statistical differences between them. Eosinophil cation protein (ECP), myeloperoxidase (MPO), IL-25, IL-33, IL-5, IL-13, interferon (IFN)-γ and IL-17 were increased in the cultures, however, only concentrations of MPO, IFN-γ and IL-17 were enhanced in CRSwNP tissues compared to CRSsNP ones. After administration of rmIL-33, ECP, IL-5 and IL-13 were all increased in tissues from CRSsNP and CRSwNP patients, however, there were no significant differences between them. Finally, we evaluated concentrations of several above inflammatory factors after the treatment of rmIL-17, and found that MPO and IFN-γ were enhanced in these two phenotypes of patients, and were elevated significantly in CRSwNP tissue cultures.

Conclusion

These findings show that ILC2s might be inactivated in neutrophilic CRSsNP and CRSwNP based on this pilot study.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 81371076), and the Shanghai Suburb Tertiary Hospital Clinical Capacity Building Project (Grant No. SHDC12015905).

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Huashan Hospital of Fudan University, No. 12 Wulumuqi Middle Road, Shanghai, 200040, China

    Lin Lin, Jinjin Wei, Zheng Chen, Xinyue Tang, Fei Dai & Guangbin Sun

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  1. Lin Lin
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  2. Jinjin Wei
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  3. Zheng Chen
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  4. Xinyue Tang
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Correspondence to Lin Lin.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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For this type of study formal consent is not required. This article does not contain any studies with animals performed by any of the authors.

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Lin, L., Wei, J., Chen, Z. et al. Activations of group 2 innate lymphoid cells depend on endotypes of chronic rhinosinusitis. Eur Arch Otorhinolaryngol 275, 3007–3016 (2018). https://doi.org/10.1007/s00405-018-5180-4

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  • DOI: https://doi.org/10.1007/s00405-018-5180-4

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