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Expression of matrix metalloproteinases (MMPs-2, -7, -9, and -26) and tissue inhibitors of metalloproteinases (TIMPs-1 and -2) in pleomorphic adenomas and adenoid cystic carcinomas

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Abstract

Purpose

To compare the immunohistochemical expression of matrix metalloproteinases-2, -7, -9 and -26 and tissue inhibitors of metalloproteinases-1 and -2 in pleomorphic adenomas and adenoid cystic carcinomas of the minor salivary glands.

Methods

Twenty cases of pleomorphic adenomas and 20 cases of adenoid cystic carcinomas were evaluated for the immunohistochemical expression of matrix metalloproteinases-2, -7, -9, and -26 and tissue inhibitors-1 and -2 in tumor parenchyma.

Results

Most pleomorphic adenomas and adenoid cystic carcinomas showed high expression of matrix metalloproteinases and tissue inhibitors, predominantly located in the tumor cells. There was no statistically significant difference in the expression of the metalloproteinases-2 (p = 0.359), -7 (p = 0.081), and -26 (p = 0 553), as well as the tissue inhibitors-1 (p = 0.657), and -2 (p = 0.248) between the parenchyma of the studied tumors. Only matrix metalloproteinase-9 showed a significant difference in expression between the two tumors, with adenoid cystic carcinoma showing a more intense staining for this gelatinase (p = 0.041).

Conclusions

The expression of the studied metalloproteinases suggests the involvement of these enzymes in the tissue remodeling process in pleomorphic adenomas and adenoid cystic carcinomas, but only MMP-9, significantly expressed in the adenoid cystic carcinomas, appears to be involved in the process of invasiveness and more aggressive behavior of these tumors. Additionally, results point that TIMPs-1 and -2 may have more complex functions besides metalloproteinase inhibition, which may be related to the pathogenesis and biological behavior of salivary gland tumors.

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Funding

Supported by CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant no. 307539/2010-5). Jean Nunes dos Santos, Cassiano Francisco Weege Nonaka, Leão Pereira Pinto and Lelia Batista de Souza are research fellows at CNPq, Brazil.

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Authors and Affiliations

  1. Department of Health Sciences, Oral Cancer Research Group, State University of Feira de Santana, Feira de Santana, Bahia, Brazil

    Valéria Souza Freitas

  2. Laboratory of Oral Surgical Pathology, Department of Oral Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil

    Jean Nunes dos Santos

  3. Oral Pathology Post-Graduation Program, Federal University of Rio Grande do Norte, Av. Senador Salgado Filho, 1787, Lagoa Nova, Natal, Rio Grande Do Norte, 59056-000, Brazil

    Pedro Paulo de Andrade Santos, Leão Pereira Pinto & Lélia Batista de Souza

  4. Department of Dentistry, State University of Paraíba, Campina Grande, Paraíba, Brazil

    Cassiano Francisco Weege Nonaka

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  1. Valéria Souza Freitas
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Correspondence to Lélia Batista de Souza.

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This study was approved by the UFRN Research Ethics Committee, Natal, Brazil (No. 203/2008).

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Certify that all authors (Valéria Souza Freitas; Jean Nunes dos Santos; Pedro Paulo de Andrade Santos; Cassiano Francisco Weege Nonaka; Leão Pereira Pinto and Lélia Batista de Souza) have viewed and agreed to the manuscript submitted.

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Souza Freitas, V., dos Santos, J.N., de Andrade Santos, P.P. et al. Expression of matrix metalloproteinases (MMPs-2, -7, -9, and -26) and tissue inhibitors of metalloproteinases (TIMPs-1 and -2) in pleomorphic adenomas and adenoid cystic carcinomas. Eur Arch Otorhinolaryngol 275, 3075–3082 (2018). https://doi.org/10.1007/s00405-018-5176-0

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