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Mitochondrial DNA deletions in patients with chronic suppurative otitis media

  • Otology
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Abstract

The aim of this study was to investigate the 4977 and 7400 bp deletions of mitochondrial DNA in patients with chronic suppurative otitis media and to indicate the possible association of mitochondrial DNA deletions with chronic suppurative otitis media. Thirty-six patients with chronic suppurative otitis media were randomly selected to assess the mitochondrial DNA deletions. Tympanomastoidectomy was applied for the treatment of chronic suppurative otitis media, and the curettage materials including middle ear tissues were collected. The 4977 and 7400 bp deletion regions and two control regions of mitochondrial DNA were assessed by using the four pair primers. DNA was extracted from middle ear tissues and peripheral blood samples of the patients, and then polymerase chain reactions (PCRs) were performed. PCR products were separated in 2 % agarose gel. Seventeen of 36 patients had the heterozygote 4977 bp deletion in the middle ear tissue but not in peripheral blood. There wasn’t any patient who had the 7400 bp deletion in mtDNA of their middle ear tissue or peripheral blood tissue. The patients with the 4977 bp deletion had a longer duration of chronic suppurative otitis media and a higher level of hearing loss than the others (p < 0.01). Long time chronic suppurative otitis media and the reactive oxygen species can cause the mitochondrial DNA deletions and this may be a predisposing factor to sensorineural hearing loss in chronic suppurative otitis media. An antioxidant drug as a scavenger agent may be used in long-term chronic suppurative otitis media.

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Acknowledgments

We thank Prof. Hasan Turkez, PhD, from Erzurum Technical University, for statistical analysis of this study.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    Arzu Tatar & Ozgur Yoruk

  2. Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    Sener Tasdemir, Ibrahim Sahin, Haktan Bagis Erdem & Abdulgani Tatar

  3. Department of Molecular Biology, Faculty of Science, Ataturk University, Erzurum, Turkey

    Ceyda Bozoglu

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  1. Arzu Tatar
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  2. Sener Tasdemir
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  3. Ibrahim Sahin
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  4. Ceyda Bozoglu
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Correspondence to Arzu Tatar.

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Tatar, A., Tasdemir, S., Sahin, I. et al. Mitochondrial DNA deletions in patients with chronic suppurative otitis media. Eur Arch Otorhinolaryngol 273, 2473–2479 (2016). https://doi.org/10.1007/s00405-015-3839-7

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  • DOI: https://doi.org/10.1007/s00405-015-3839-7

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