Abstract.
The semiquantitative reverse transcription polymerase chain reaction was employed to detect the expression of transforming growth factor β (TGF-β) and insulin-like growth factor (IGF) in complete hydatidiform mole, normal first-trimester villi, the normal term placenta (after vaginal/abdominal deliver) and the preeclamptic placenta at term. The expression of IGF-I mRNA was seen in all five tissues, but its level was much lower in the term placental tissues with preeclampsia than in other tissues. The content of IGF-I mRNA in villous tissues from molar pregnancy was slightly higher than in normal first-trimester villi. IGF-II mRNA was detected at similar levels in all three sorts of term placental tissues. However, the expression level of IGF-II mRNA in tissues of complete molar pregnancy was significantly lower than in normal first-trimester villi. TGF-β3 was found expressed in all five tissues, while TGF-β1 and TGF-β2 mRNA were not detected. Compared to the normal first-trimester villi, the expression of TGF-β3 in complete hydatidiform molar tissues was comparatively higher. Furthermore, the expression levels of TGF-β3 in the preeclamptic placenta and the normal placenta after cesarean birth were higher than in the placenta after vaginal delivery. We concluded that, the change of TGF-β and IGF expression in placental tissues might be involved in the development of trophoblastic diseases of pregnancy.
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This work was one part of Project 973, supported by the Natural Science Foundation of China (1-G1999055903).
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Pang, ZJ., Xing, FQ. Expression of transforming growth factor-β and insulin-like growth factor in molar and placental tissues. Arch Gynecol Obstet 269, 1–4 (2003). https://doi.org/10.1007/s00404-002-0379-3
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DOI: https://doi.org/10.1007/s00404-002-0379-3