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Inactivated P. aeruginosa restores immune imbalance of chronic idiopathic urticaria

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Abstract

The main pathology involved in chronic idiopathic urticaria (CIU) is immunological dysfunction which mainly adapts to the immune system of body. Pseudomonas aeruginosa-mannose-sensitive hemagglutinin (PA) is an inactivated Pseudomonas aeruginosa biological product which displays a broad immune regulatory effect. The current study was designed to explore the protective nature of PA as an immune regulator in CIU. The participants were randomly divided into CIU + PA, CIU, control + PA and control group. lg E, anti FcεRI, anti IgE antibody, IL-4, IL-17, TGF-β1 and interferon-γ in the sera were assayed by ELISA. Then CD4+ T cells and CD19+ B cells were isolated from peripheral blood of patients with CIU (n = 10) and healthy control (n = 10). CD4+ T cells and CD19+ B proliferation and apoptosis were analyzed through CCK-8 and flow cytometry respectively. T helper cells differentiations were assessed by real-time PCR. The results revealed that compared with the control group, the curative effect of CIU + PA group was more effective than that of the CIU control group. There was a hyper proliferation of CD19+ B cells in the CIU patients. Moreover, it was also discovered that presence of Th1 decreased while Th2 cells increased in CIU patients. PA significantly inhibited the proliferation of CD19+ B and Th2 cells but at the same time promoted the proliferation of Th1 compared to healthy control. The conclusion arrived at from this study is that the PA displayed a remarkable regulatory effect in CD4+ T cells and CD19+ B cells function by promoting Th1 but inhibited Th2 and the hyperfunction of B cells of CIU patients.

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Authors and Affiliations

  1. Department of Pharmacy, Shandong Provincial Third Hospital, 12 Wuyingshan Middle Road, Tianqiao District, Jinan, 250031, Shandong, China

    Xie Jian, Song Chao & Zhang Xiaoli

  2. Department of Pharmacy, Shandong Provincial First Hospital, Jinan, 250021, Shandong, China

    Wang Aiwu

Authors
  1. Xie Jian
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  2. Song Chao
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  3. Zhang Xiaoli
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  4. Wang Aiwu
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Contributions

All authors participated in the design, interpretation of the studies and analysis of the data and review of the manuscript; XJ, SC, ZX and WA conducted the experiment. SC wrote the manuscript.

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Correspondence to Song Chao.

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The author(s) declare that they have no conflict of interest.

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The study was approved by the Institutional Ethic Committee of No.3 Hospital of Shandong (3SDH-2016-190).

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Informed consent was obtained from all individual participants included in the study.

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Jian, X., Chao, S., Xiaoli, Z. et al. Inactivated P. aeruginosa restores immune imbalance of chronic idiopathic urticaria. Arch Dermatol Res 312, 353–359 (2020). https://doi.org/10.1007/s00403-019-02019-3

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  • DOI: https://doi.org/10.1007/s00403-019-02019-3

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