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Development and evaluation of a vegetable oil blend formulation for cutaneous wound healing

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Abstract

This work aimed to evaluate the in vivo capacity of a vegetable oil blend formulation (VOB) developed to accelerate cutaneous wound closure. Total thickness wounds were punctured on the skin on the back side of each animal and topically treated with the VOB formulation, Dersani® ointment or the vehicle control. After 2, 7, 14, 21 days post-wounding, five animals from each group were euthanized, and the rates of wound closure and re-epithelialization were evaluated. The wounds were harvested for histological and biochemical analysis. VOB resulted in faster and greater re-epithelialization in the in vivo excisional wounds, exhibiting significant wound area reduction of 8.9, 8.0, 35.1, 45.2 and 47.0% after 2, 5, 10, 14 and 21 days post-wounding, respectively, when compared with the vehicle control. Histological and biochemical analyses showed that the VOB-treated wounds exhibited a significant increase of granular tissue and controlled inflammatory response by modulation of the release of pro-inflammatory cytokines TNF-α, IL-6 and IL-1. Moreover, VOB-treated wounds showed a significant and concrete increase in the deposition and organisation of collagen fibres in the wound site and improved the quality of the scar tissue. Altogether, these data revealed that VOB accelerates wound healing processes and might be beneficial for treating wound disorders.

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Acknowledgements

The authors wish to thank the Fundação de Amparo à Pesquisa do Espirito Santo (FAPES), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Produtos Naturais, Universidade Vila Velha-UVV, Av. Comissário José Dantas de Melo, no. 21, Boa Vista, Vila Velha, Espírito Santo, 29102-920, Brazil

    Marcio Guidoni, Mariana Moreira Figueira, Gabrielly Pereira Ribeiro, Dominik Lenz, Thiago de Melo Costa Pereira, Rodrigo Scherer & Marcio Fronza

  2. Instituto de Química de São Carlos-IQSC, Universidade de São Paulo-USP, São Carlos, São Paulo, Brazil

    Pamela Aparecida Grizotto & Stanislau Bogusz Jr.

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  1. Marcio Guidoni
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  2. Mariana Moreira Figueira
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  3. Gabrielly Pereira Ribeiro
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  5. Pamela Aparecida Grizotto
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  6. Thiago de Melo Costa Pereira
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Correspondence to Marcio Fronza.

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Ethical standards

This work follows ethical standards and all experiments involving the use of animals were conducted in agreement with the Brazilian Animal Care Committee and were approved by the Committee of Ethics, Bioethics and Animal Welfare of the Universidade Vila Velha (UVV) (CEUA-UVV protocol 381/2016).

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Guidoni, M., Figueira, M.M., Ribeiro, G.P. et al. Development and evaluation of a vegetable oil blend formulation for cutaneous wound healing. Arch Dermatol Res 311, 443–452 (2019). https://doi.org/10.1007/s00403-019-01919-8

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  • DOI: https://doi.org/10.1007/s00403-019-01919-8

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