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Cytokine polymorphisms in patients with pemphigus

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Abstract

The aim of this study was to assess whether tumor necrosis factor α (TNF-α), transforming growth factor β1 (TGF-β1) and interleukin-10 (IL-10) polymorphisms are among the factors influencing the development of pemphigus. Whole blood from 20 patients with pemphigus and 24 control subjects was taken. Genomic DNA was obtained and cytokine genotyping for IL-10 (−1082 G/A; −819 C/T), TGFB1 (codon 10 C/T, codon 25 G/C) and TNFA (−308 G/A) was performed using the ARMS-PCR method. The distribution of IL-10 (−819) alleles was significantly different between the pemphigus and control groups (P=0.009). In particular, allele T was associated with the disease (OR 3.291, 95% CI 1.350–8.020). Similar results were observed when only pemphigus vulgaris (PV) patients were analyzed (P=0.012, OR 3.410, 95% CI 1.346–8.639). An increased frequency of the low producer IL-10 haplotype (−1082/−819 A/T) in patients with pemphigus compared with controls was observed (OR 2.714, 95% CI 1.102–6.685) and this association was also significant when only PV patients were considered (OR 2.667, 95% CI 1.043–6.816). There were no differences between patients and controls in the frequency of any other gene polymorphism analyzed. The increased frequency of the low producer IL-10 haplotype (−1082 /−819 A/T) suggest that the carriage of this haplotype might predispose to pemphigus or the high and intermediate producer haplotypes may be protective factors. The prevalence of the allele IL-10 (−819 T) in pemphigus patients cannot be explained by the current hypothesis, according to which a particular allele of the gene is associated with a different level of cytokine production and therefore affects the predisposition to a particular disease. However, this cytokine polymorphism might be linked to an unknown susceptibility factor.

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Acknowledgements

Agencia Cordoba Ciencia and SECYT/UNC from Argentina partially supported this work. CONICET from Argentina supports Yanina Eberhard.

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Authors and Affiliations

  1. Immunology, Clinical Biochemistry, School of Chemical Science, National University of Cordoba, Haya de la Torre esquina, Medina Allende Ciudad Universitaria, Cordoba, 5000, Argentina

    Yanina Eberhard & Horacio Marcelo Serra

  2. Dermatology Department, National Clinical Hospital, National University of Cordoba, Cordoba, Argentina

    Elisa Burgos

  3. Allergy Department, Private Hospital, Cordoba, Argentina

    Julio Gagliardi

  4. Immunogenetic and Molecular Diagnostic Laboratory (LIDMO), Cordoba, Argentina

    Carlos Maria Vullo, Alicia Borosky & Susana Pesoa

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  1. Yanina Eberhard
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  2. Elisa Burgos
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  3. Julio Gagliardi
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  7. Horacio Marcelo Serra
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Correspondence to Horacio Marcelo Serra.

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Eberhard, Y., Burgos, E., Gagliardi, J. et al. Cytokine polymorphisms in patients with pemphigus. Arch Dermatol Res 296, 309–313 (2005). https://doi.org/10.1007/s00403-004-0528-6

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  • DOI: https://doi.org/10.1007/s00403-004-0528-6

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