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Acute supplementation with grapes in obese subjects did not affect postprandial metabolism: a randomized, double-blind, crossover clinical trial

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Abstract

Purpose

The aim of this study was to determine whether grape polyphenols have a “second-meal effect”, modulating glucose and lipid elevations in the postprandial period after two successive meals in subjects with obesity.

Methods

A randomized, double-blind, placebo-controlled, acute clinical trial was conducted. Twenty-five obese subjects (BMI =  ≥ 30 and < 40 kg/m2) were randomly divided into two groups. At an initial visit, blood was collected in a fasting state and the subjects received breakfast and 46 g of either grape powder (equivalent to 252 g fresh grapes) or placebo, both solved in water. Lunch was provided 5 h later and then blood was collected after 0, 30, 60, 120, 180, 240, 300, 330, 360, and 420 min since arrival. Two weeks later, at a second visit, the subjects received the other powder. The following were determined: glucose, insulin, triglycerides, uric acid, blood count, hemoglobin, viscosity, antioxidant capacity, and satiety perception.

Results

Postprandial increases were observed as expected in, for example, glucose and triglycerides after breakfast and lunch. The grape powder supplementation did not cause any significant modification compared to placebo, in these parameters; nor did it significantly modify plasma antioxidant capacity in the 6 h postprandial period.

Discussion

Single grape powder supplementation did not modify postprandial responses in obese subjects, probably because the polyphenol dose was insufficient to induce such an effect. The result of a combination of grape with other polyphenol-rich products or chronic supplementation with grape powder on postprandial responses remains to be elucidated.

Trial registration number

www.clinicaltrials.gov, NCT03741218.

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Acknowledgements

This study was funded by the California Table Grape Commission (Health Research Grants 2018). E.G.-D. was a recipient of a JAE-Intro fellowship from CSIC. E.G.-M. was the recipient of a Development of Industrial Doctorate project from the Community of Madrid (IND2018/BIO-9554) and M.M.-S. was the recipient of a contract from the European Youth Employment Initiative (Ref: CAMPD17_ICTAN_003). Miss Anastasia Palatzidi (recipient of an Erasmus+ fellowship), Miss Andrea Almagro-Soria and Mr. Enrique Báez-García (recipient of a Beca Mixta CONACYT, ref. 701313) are thanked for technical assistance during the preparation and development of the clinical trial or in biochemical determinations. Language revision by Dr. Christopher Evans is appreciated. The authors are grateful to the volunteers for their participation in the study.

Funding

This study was funded by the California Table Grape Commission (Health Research Grants 2018). E.G.-M. was the recipient of a Development of Industrial Doctorate project from the Community of Madrid (IND2018/BIO-9554) and M. Martínez-Suárez was the recipient of a contract from the European Youth Employment Initiative (Ref: CAMPD17_ICTAN_003).

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Author notes

  1. Beatriz Herranz

    Present address: Department of Food Technology, Veterinary Faculty, Complutense University, Avda/Puerta de Hierro, s/n, 28040, Madrid, Spain

Authors and Affiliations

  1. Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), José Antonio Novais 10, 28040, Madrid, Spain

    Esther García-Díez, Marta Cuesta-Hervás, Ana M. Veses-Alcobendas, Óscar Alonso-Gordo, Elena García-Maldonado, Miriam Martínez-Suárez, M. Pilar Vaquero & Jara Pérez-Jiménez

  2. Department of Characterization, Quality, and Safety, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), Madrid, Spain

    Beatriz Herranz & María Dolores Álvarez

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  1. Esther García-Díez
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Contributions

JP-J conceived the study, got funding and supervised all tasks; MC-H, OA-G, EG-M, JP-J and AMV-A performed volunteer recruitment and the development of the clinical trial; EG-M, OA-G and MM-S processed biological samples; MDÁ, MC-H, OA-G, EG-D, BH and AMV-A performed biochemical determinations in biological samples; EG-D carried out data curation, statistical analysis and visualization; MDÁ, EG-D, JP-J and MPV interpreted the results; JP-J wrote the first draft of the manuscript. The manuscript was reviewed and approved by all authors.

Corresponding author

Correspondence to Jara Pérez-Jiménez.

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García-Díez, E., Cuesta-Hervás, M., Veses-Alcobendas, A.M. et al. Acute supplementation with grapes in obese subjects did not affect postprandial metabolism: a randomized, double-blind, crossover clinical trial. Eur J Nutr 60, 2671–2681 (2021). https://doi.org/10.1007/s00394-020-02451-6

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  • DOI: https://doi.org/10.1007/s00394-020-02451-6

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