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The effect of APTR, Fn14 and CD133 expressions on liver fibrosis in biliary atresia patients

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Abstract

Purpose

Although some biomarkers of hepatic progenitor cells have been reported to be involved in the liver fibrosis in patients with biliary atresia (BA), however, research still shows conflicting results. Therefore, we investigated the effect of Alu-mediated p21 transcriptional regulator (APTR), fibroblast growth factor-inducible 14 (Fn14) and CD133 expressions on liver fibrosis in Indonesian BA patients.

Methods

Nineteen liver samples from BA patients and 9 liver specimens from non-BA controls were obtained. The expressions of APTR, Fn14 and CD133 were analyzed by quantitative real-time polymerase chain reaction (qPCR).

Results

APTR expression was strongly up-regulated (1.5-fold) in liver BA specimens compared to liver controls (ΔCT 3.2 ± 0.6 vs 3.8 ± 0.51; p = 0.028). Moreover, Fn14 and CD133 expressions were similar in the BA and control groups (ΔCT 2.7 ± 1.3 vs. 1.4 ± 1.6, p = 0.07; and 12.0 ± 3.7 vs. 11.78 ± 2.30, p = 0.88, respectively). Intriguingly, CD133 expression was strongly related with the survival of BA patients (p = 0.0061), but not with age at Kasai procedure (p = 0.36) and the presence of cirrhosis (p = 0.77).

Conclusion

We present the first study of aberrant APTR expressions in the liver of BA infants which might contribute to liver fibrogenesis in BA infants.

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Acknowledgements

We want to give thanks to everybody who assisted the study. We are also thankful to an English editing service staff at the Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada (FK-KMK UGM) for checking the manuscript grammar.

Funding

This study was funded by the FK-KMK UGM (Grant nos. UPPM/220/M/05/04/05.17 and UPPM/363/M/05/04/05.18 to G and AM).

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Authors and Affiliations

  1. Pediatric Surgery Division, Department of Surgery/Genetics Working Group, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Jl. Kesehatan No. 1, Yogyakarta, 55281, Indonesia

    Akhmad Makhmudi, Reinaldo Supanji, Bayu Pratama Putra &  Gunadi

Authors
  1. Akhmad Makhmudi
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  2. Reinaldo Supanji
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  3. Bayu Pratama Putra
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  4. Gunadi
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Corresponding author

Correspondence to Gunadi.

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Conflict of interest

The authors declared no potential conflicts of interest.

Ethical approval

This study has an approval from the Ethical Committee of the FK-KMK UGM/Dr. Sardjito Hospital (KE/FK/1252/EC/2018 and KE/FK/1088/EC/2017).

Informed consent

Written informed consent was obtained from the parents’ BA patients.

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Makhmudi, A., Supanji, R., Putra, B.P. et al. The effect of APTR, Fn14 and CD133 expressions on liver fibrosis in biliary atresia patients. Pediatr Surg Int 36, 75–79 (2020). https://doi.org/10.1007/s00383-019-04582-2

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