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Effect of long-term fertilization on bacterial composition in rice paddy soil

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Abstract

We investigated the effect of long-term fertilization on bacterial abundance, composition, and diversity in paddy soil. The experiment started in 1990 in Taoyuan Agro-ecosystem Research Station in China (111°33′ E, 28°55′ N). The molecular approaches including real-time quantitative PCR, terminal restriction fragment length polymorphism, and clone library construction were employed using 16S rRNA gene as genetic marker. Application of inorganic fertilizers did not affect bacterial abundance, and rice straw incorporation combined with inorganic fertilizers significantly (P < 0.05) increased bacterial abundance with shifts in bacterial community composition. Among phylogenetic groups, γ-Proteobacteria was responsive to all fertilization regimes while Acidobacteria was relatively stable to fertilization practices. Inorganic fertilizer mainly affected γ-Proteobacteria and δ-Proteobacteria, while rice straw incorporation influenced β-Proteobacteria and Verrucomicrobia. Therefore, long-term fertilization can affect abundance and composition of bacterial communities in paddy soil.

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Acknowledgments

This work was financially supported by the Chinese Academy of Sciences (KZCX2-YW-BR-01, KZCX2-YW-T07, and KZCX2-YW-423) and the Natural Science Foundation of China (40771115, 40901125). We thank Prof. Paolo Nannipieri for careful revision of this manuscript.

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  1. Minna Wu

    Present address: Xinxiang Medical University, Xinxiang, Henan, 450003, China

  2. Zhe Chen

    Present address: State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry (LAPC), Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, 100029, China

Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Mapoling, ChangSha, 410125, China

    Minna Wu, Hongling Qin, Zhe Chen, Jinshui Wu & Wenxue Wei

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  1. Minna Wu
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Correspondence to Wenxue Wei.

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Wu, M., Qin, H., Chen, Z. et al. Effect of long-term fertilization on bacterial composition in rice paddy soil. Biol Fertil Soils 47, 397–405 (2011). https://doi.org/10.1007/s00374-010-0535-z

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