Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta)

Abstract

Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive Mg²⁺ load from the environment with an equal excretion. However, the molecular transporters involved in Mg²⁺ secretion are poorly understood. We investigated whether environmental MgCl₂ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal Mg²⁺ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the Na⁺/K⁺-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol L⁻¹ ambient [Mg²⁺], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [Mg²⁺] was explored by exposing toadfish to control (50 mmol L⁻¹ Mg²⁺), or elevated [Mg²⁺] (100 mmol L⁻¹) at a constant salinity for 7 days. Mg²⁺ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In Mg²⁺-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [Mg²⁺], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal Mg²⁺ transporters is induced by elevated [Mg²⁺] in combination with salinity rather than elevated ambient [Mg²⁺] alone.