Abstract
We describe here a set of genetic markers, based on IRS-PCR amplification difference, that are specifically designed for efficient, high throughput genetic mapping in [(M. domesticus × wild-derived) F1 × M. domesticus] interspecific backcrosses. 146 new genetic loci have been mapped, and strain distribution for these markers has been determined in 96 mouse strains. 103 (81%) of 127 tested markers are present only in one or more wild-derived strains, but absent in 76 other commonly used strains, demonstrating their utility in a variety of mouse pair combinations. Because of the ease of genotyping with this marker set, rapid genome scans for complex genetic trait loci involving crosses between wild-derived strains and other commonly used strains can now be carried out efficiently with large numbers of animals.
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Elango, R., Riba, L., Housman, D. et al. Generation and mapping of Mus spretus strain-specific markers for rapid genomic scanning. Mammalian Genome 7, 340–343 (1996). https://doi.org/10.1007/s003359900099
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DOI: https://doi.org/10.1007/s003359900099