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Adaptation of root growth to increased ambient temperature requires auxin and ethylene coordination in Arabidopsis

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Abstract

Key message

A fresh look at the roles of auxin, ethylene, and polar auxin transport during the plant root growth response to warmer ambient temperature (AT).

Abstract

The ambient temperature (AT) affects plant growth and development. Plants can sense changes in the AT, but how this change is transduced into a plant root growth response is still relatively unclear. Here, we found that the Arabidopsis ckrc1-1 mutant is sensitive to higher AT. At 27 °C, the ckrc1-1 root length is significantly shortened and the root gravity defect is enhanced, changes that can be restored with addition of 1-naphthaleneacetic acid, but not indole-3-acetic acid (IAA). AUX1, PIN1, and PIN2 are involved in the ckrc1-1 root gravity response under increased AT. Furthermore, CKRC1-dependent auxin biosynthesis was critical for maintaining PIN1, PIN2, and AUX1 expression at elevated temperatures. Ethylene was also involved in this regulation through the ETR1 pathway. Higher AT can promote CKRC1-dependent auxin biosynthesis by enhancing ETR1-mediated ethylene signaling. Our research suggested that the interaction between auxin and ethylene and that the interaction-mediated polar auxin transport play important roles during the plant root growth response to higher AT.

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Abbreviations

AT:

Ambient temperature

AVG:

Aminoethoxyvinylglycine

ACC:

1-Aminocyclopropane-1-carboxylic acid

ckrc1 :

CK-induced root curling 1

CK:

Cytokinin

d:

Day(s)

wei8 :

Ethylene insensitive 8

NAA:

1-Naphthaleneacetic acid

IAA:

Indole-3-acetic acid

taa1 :

Tryptophan Aminotransferase of Arabidopsis 1

yuc8 :

yucca8

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Acknowledgements

Thanks to the Core Facility of School of Life Sciences, Lanzhou University. Prof Guangqin Guo provided experimental space, necessary material, and some discussion. Lei Wu provided help in some experiments.

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Author notes

  1. Shaodong Wei

    Present address: Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark

  2. Qionghui Fei and Shaodong Wei have contributed equally to this article.

Authors and Affiliations

  1. Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China

    Qionghui Fei, Shaodong Wei, Huanhuan Gao & Xiaofeng Li

  2. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

    Zhaoyang Zhou

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  1. Qionghui Fei
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Correspondence to Xiaofeng Li.

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The authors declare that they have no conflict of interest.

Funding

This work was supported by Grants from the Chinese National Science Foundation (31201062), the Fundamental Research Funds for the Central Universities (lzujbky-2014-199, lzujbky-2016-88), and the foundation of the Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education (lzujbky-2013-bt05). The funding sources had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Communicated by Prakash P. Kumar.

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Fei, Q., Wei, S., Zhou, Z. et al. Adaptation of root growth to increased ambient temperature requires auxin and ethylene coordination in Arabidopsis . Plant Cell Rep 36, 1507–1518 (2017). https://doi.org/10.1007/s00299-017-2171-7

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  • DOI: https://doi.org/10.1007/s00299-017-2171-7

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