Abstract
Transgenic Gladiolus plants that contain either Cucumber mosaic virus (CMV) subgroup I coat protein, CMV subgroup II coat protein, CMV replicase, a combination of the CMV subgroups I and II coat proteins, or a combination of the CMV subgroup II coat protein and replicase genes were developed. These plants were multiplied in vitro and challenged with purified CMV isolated from Gladiolus using a hand-held gene gun. Three out of 19 independently transformed plants expressing the replicase gene under control of the duplicated CaMV 35S promoter were found to be resistant to CMV subgroup I. Three out of 21 independently transformed plants with the CMV subgroup II coat protein gene under control of the Arabidopsis UBQ3 promoter were resistant to CMV subgroup II. Eighteen independently transformed plants with either the CMV subgroup I coat protein or a combination of CMV subgroups I and II coat proteins were challenged and found to be susceptible to both CMV subgroups I or II. Virus resistant plants with the CMV replicase transgene expressed much lower RNA levels than resistant plants expressing the CMV subgroup II coat protein. This work will facilitate the evaluation of virus resistance in transgenic Gladiolus plants to yield improved floral quality and productivity.
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Acknowledgments
Mention of a trademark, proprietary product or vendor does not imply its approval to the exclusion of other products or vendors that may also be suitable. The following people at the USDA are thanked for their assistance. Charity James challenged the plants. Anne O’Connor prepared the DNA blots for Southern hybridization. Dr. Slava Andrianov performed PCR on the putatively transformed plants for detection of the transgenes. This project was funded in part by BARD project number IS-2760-96 awarded to K. Kamo, H. T. Hsu, and P. Ueng.
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Communicated by P. Ozias-Akins.
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Kamo, K., Jordan, R., Guaragna, M.A. et al. Resistance to Cucumber mosaic virus in Gladiolus plants transformed with either a defective replicase or coat protein subgroup II gene from Cucumber mosaic virus . Plant Cell Rep 29, 695–704 (2010). https://doi.org/10.1007/s00299-010-0855-3
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DOI: https://doi.org/10.1007/s00299-010-0855-3