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Interactions between RNA-binding proteins and P32 homologues in trypanosomes and human cells

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Abstract

RNA-binding proteins (RBPs) are involved in many aspects of mRNA metabolism such as splicing, nuclear export, translation, silencing, and decay. To cope with these tasks, these proteins use specialized domains such as the RNA recognition motif (RRM), the most abundant and widely spread RNA-binding domain. Although this domain was first described as a dedicated RNA-binding moiety, current evidence indicates these motifs can also engage in direct protein–protein interactions. Here, we discuss recent evidence describing the interaction between the RRM of the trypanosomatid RBP UBP1 and P22, the homolog of the human multifunctional protein P32/C1QBP. Human P32 was also identified while performing a similar interaction screening using both RRMs of TDP-43, an RBP involved in splicing regulation and Amyotrophic Lateral Sclerosis. Furthermore, we show that this interaction is mediated by RRM1. The relevance of this interaction is discussed in the context of recent TDP-43 interactomic approaches that identified P32, and the numerous evidences supporting interactions between P32 and RBPs. Finally, we discuss the vast universe of interactions involving P32, supporting its role as a molecular chaperone regulating the function of its ligands.

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Acknowledgments

We apologize to all the colleagues whose work could not be cited in this article due to space limitations. The work described here was performed with financial support from the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) to AC. AC is a member of the Research Career of CONICET, and GC is a CONICET Research Fellow. The funders had no role in study design, data collection and analysis, and decision to publish or preparation of the manuscript.

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  1. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, UNSAM-CONICET, Buenos Aires, Argentina

    Juan Manuel Polledo, Gabriela Cervini, María Albertina Romaniuk & Alejandro Cassola

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  1. Juan Manuel Polledo
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  2. Gabriela Cervini
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  3. María Albertina Romaniuk
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  4. Alejandro Cassola
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Correspondence to Alejandro Cassola.

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Communicated by M. Kupiec.

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Polledo, J.M., Cervini, G., Romaniuk, M.A. et al. Interactions between RNA-binding proteins and P32 homologues in trypanosomes and human cells. Curr Genet 62, 203–212 (2016). https://doi.org/10.1007/s00294-015-0519-5

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  • DOI: https://doi.org/10.1007/s00294-015-0519-5

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