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Prevalence of qnrVC Genes in Pseudomonas aeruginosa Clinical Isolates from Guangdong, China

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Abstract

Pseudomonas aeruginosa is a serious nosocomial pathogen with high morbidity and mortality due to the increasing resistance to antibiotics in recent years. qnrVC genes have been proven as a source of antibiotic resistance, but relationship with Pseudomonas aeruginosa remains not clear. We aimed to investigate the prevalence and molecular characteristics of qnrVC genes in P. aeruginosa clinical isolates. A total of 874 nonduplicate clinical isolates were collected in Guangdong, China, between January 2011 and June 2015. The presence of qnrVC genes and their genotypes were determined using PCR amplification and DNA sequencing. Antibiotic susceptibilities were tested, and the genetic relatedness of qnrVC-positive isolates were analyzed by multi-locus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE). Consequently, we found 2.3% of P. aeruginosa isolates were present with qnrVC genes, displaying more resistant to various antibiotics. Phylogenetic analysis of qnrVC-positive strains revealed that antibacterial resistance among qnrVC-positive P. aeruginosa isolates in Guangdong probably emerged from multiple sources and was not spread by clonal strains.

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Acknowledgments

This work is supported by the research Grants from the Natural Science Foundation of China (No. 81974318), Guangdong Province Science and Technology Innovation Strategy Special Fund (No. 2019B020209001), Natural Science Foundation of Guangdong Province (Nos. 2018A030310170 and 2018A030313279) and the Guangdong Bureau of Traditional Chinese Medicine (No. 20191206).

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  1. Jinqiong Lin and Ding-Qiang Chen contributed equally to this work.

Authors and Affiliations

  1. Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China

    Jinqiong Lin

  2. Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China

    Ding-Qiang Chen

  3. KingMed College of Laboratory Medicine, Guangzhou Medical University, Guangzhou, China

    Jianming Hong, Huanhuan Huang & Xia Xu

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  1. Jinqiong Lin
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  2. Ding-Qiang Chen
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  5. Xia Xu
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Correspondence to Xia Xu.

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Lin, J., Chen, DQ., Hong, J. et al. Prevalence of qnrVC Genes in Pseudomonas aeruginosa Clinical Isolates from Guangdong, China. Curr Microbiol 77, 1532–1539 (2020). https://doi.org/10.1007/s00284-020-01974-9

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  • DOI: https://doi.org/10.1007/s00284-020-01974-9

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