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Characterization and Genome Analysis of a Novel Alteromonas Phage JH01 Isolated from the Qingdao Coast of China

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Abstract

A novel Alteromonas phage JH01, with the host strain identified to be Alteromonas marina SW-47(T), was isolated from the Qingdao coast during the summer of 2017. Transmission electron microscopy analysis showed that phage JH01 can be categorized into the Siphoviridae family, with an icosahedral head of 62 ± 5 nm and a long contractile tail of 254 ± 10 nm. The bioinformatic analysis shows that this phage consists of a linear, double-stranded 46,500 bp DNA molecule with a GC content of 44.39%, and 58 ORFs with no tRNA genes. The ORFs are classified into four groups, including phage packaging, phage structure, DNA replication and regulation, and hypothetical protein. The phylogenetic tree, constructed using neighbor-joining analysis, shows that phage JH01 has altitudinal homology with some Vibrio and Pseudoalteromonas phage B8b. Comparative analysis reveals the high similarity between phage JH01 and phage B8b. Additionally, our study of phage JH01 provides useful information for further research on the interaction between Alteromonas phages and their hosts.

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Acknowledgements

We are grateful to the research vessel Dong Fang Hong 2, for providing the seawater samples. The research was funded by the National Natural Science Foundation of China (Grant Nos. 31500339, 41676178 and 41076088), the National Key Research and Development Program of China (Grant Nos. 2017YFA0603200, 2018YFC1406704), Marine Scientific and Technological Innovation Project Financially Supported by Pilot National Laboratory for Marine Science and Technology (Qingdao) (Grant Nos. 2018SDKJ0406-6, 2016ASKJ14), and Fundamental Research Funds for the Central University of Ocean University of China (Grant Nos. 201812002, 201762017 and 201562018).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Dongxu Wang, Yong Jiang, Shicong Xiao, Min Wang, Qian Liu, Liyang Huang, Chenglong Xue, Qi Wang, Tongtong Lin & Hongbing Shao

  2. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Yong Jiang & Min Wang

  3. Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao, 266003, China

    Yong Jiang & Min Wang

  4. Institute for Marine and Antarctic Studies, University of Tasmania, TAS, Private Bag 129, Hobart, 7001, Australia

    Andrew McMinn

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  1. Dongxu Wang
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Correspondence to Yong Jiang or Min Wang.

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Wang, D., Jiang, Y., Xiao, S. et al. Characterization and Genome Analysis of a Novel Alteromonas Phage JH01 Isolated from the Qingdao Coast of China. Curr Microbiol 76, 1256–1263 (2019). https://doi.org/10.1007/s00284-019-01751-3

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