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The Versatility of Delftia sp. Isolates as Tools for Bioremediation and Biofertilization Technologies

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Abstract

Two Pb(II)-resistant bacteria isolated from a soil containing 2,500 mg/kg of Pb were identified by 16S rRNA sequencing analysis as Delftia sp. and designated as 3C and 6C. Both isolates grew at a Pb(II) concentration of 62 mg/L and at the stationary phase showed a Pb(II)-sorption capability of 10 ± 1.5 (3C) and 5 ± 0.8 (6C) mg/g of biomass. Biochemical properties related to heavy metal resistance and plant growth promotion were analyzed and compared with the Cr(VI)-resistant plant growth-promoting Delftia sp. JD2, previously reported by our group. Both isolates and JD2 were resistant to Cr(VI), Pb(II) and many antibiotics, produced siderophores and the phytohormone indole-3-acetic, and showed clover growth-promoting activity in greenhouse conditions. Interestingly, the occurrence of integron class 1 was shown in all isolates. Our results add to previous reports and suggest that bacteria of the genus Delftia could be consider as good candidates for the design of technologies for cleaning up contaminated environments and/or the production of biofertilizers.

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Acknowledgments

We thank Programa de Desarrollo de las Ciencias Básicas (PEDECIBA-Uruguay) for partial financial support. The work of V. Braña, M. Morel, and C. Martinez were supported by Agencia Nacional de Investigación e Innovación (ANII).

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Authors and Affiliations

  1. Unit of Molecular Microbiology, Institute of Biological Research Clemente Esatble, Av. Italia 3318, Montevideo, Uruguay

    Martha C. Ubalde, Victoria Braña, Fabiana Sueiro, María A. Morel, Cecilia Martínez-Rosales & Susana Castro-Sowinski

  2. Department of Biochemistry and Molecular Biology, Faculty of Science, University of the Republic (UdelaR), Igua 4225, Montevideo, Uruguay

    Fabiana Sueiro, Cecilia Martínez-Rosales & Susana Castro-Sowinski

  3. Department of Microbiology, Faculty of Science and Faculty of Chemistry, UdelaR, Gral. Flores 2124, Montevideo, Uruguay

    Carolina Marquez

Authors
  1. Martha C. Ubalde
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  2. Victoria Braña
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  3. Fabiana Sueiro
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  4. María A. Morel
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  5. Cecilia Martínez-Rosales
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  6. Carolina Marquez
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  7. Susana Castro-Sowinski
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Corresponding author

Correspondence to Susana Castro-Sowinski.

Additional information

M.C. Ubalde and V. Braña have contributed equally to this study.

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Ubalde, M.C., Braña, V., Sueiro, F. et al. The Versatility of Delftia sp. Isolates as Tools for Bioremediation and Biofertilization Technologies. Curr Microbiol 64, 597–603 (2012). https://doi.org/10.1007/s00284-012-0108-5

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  • DOI: https://doi.org/10.1007/s00284-012-0108-5

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