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Thiosulfate Oxidation by Comamonas sp. S23 Isolated from a Sulfur Spring

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Abstract

A bacterial isolate S23 capable of oxidizing thiosulfate was isolated from a sulfur spring. Strain S23 is gram-negative, aerobic, and motile. The G + C content of DNA is 61.4 mol%. The fatty acid composition and phylogenetic analysis of the 16S rRNA gene sequence of strain S23 showed that it is related to the members of the genus Comamonas, and most closely related to Comamonas testosteroni (99.9% sequence similarity). The isolate S23 exhibited thiosulfate oxidation under a mixotrophic growth condition. Polymerase chain reaction (PCR) using soxB-specific primers and DNA sequencing showed the presence of the soxB gene. This is the first report in Comamonas sp. showing thiosulfate oxidation under a mixotrophic growth condition.

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Acknowledgments

We are grateful to Prof. R.M. Kroppenstedt and Dr. P. Schumann, DSMZ of Germany for their help in determining the G + C content and the fatty acid composition of the organism. This work was supported by the Department of Biotechnology, Ministry of Science and Technology, Government of India.

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  1. Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751 023, India

    Shachindra K. Pandey, Kunwar Digvijay Narayan, Saumya Bandyopadhyay, Kinshuk C. Nayak & Subrata K. Das

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  1. Shachindra K. Pandey
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  2. Kunwar Digvijay Narayan
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Correspondence to Subrata K. Das.

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Pandey, S.K., Narayan, K.D., Bandyopadhyay, S. et al. Thiosulfate Oxidation by Comamonas sp. S23 Isolated from a Sulfur Spring. Curr Microbiol 58, 516–521 (2009). https://doi.org/10.1007/s00284-009-9357-3

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