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Metalloid Reducing Bacteria Isolated from Deep Ocean Hydrothermal Vents of the Juan de Fuca Ridge, Pseudoalteromonas telluritireducens sp. nov. and Pseudoalteromonas spiralis sp. nov

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Abstract

Five strains of Gram-negative, rod, curved rod and spiral-shaped bacteria were isolated from the vicinity of deep ocean hydrothermal vents along the Main Endeavour Segment of the Juan de Fuca Ridge in the Pacific Ocean. All strains showed remarkable resistance to high levels of toxic metalloid oxyanions, and were capable of reducing the oxyanions tellurite and selenite to their less toxic elemental forms. Phylogenetic analysis of four strains identified these isolates as close relatives of the genus Pseudoalteromonas within the class Gammaproteobacteria. Pseudoalteromonas agarivorans was the closest relative of strains Te-1-1 and Se-1-2-redT, with, respectively, 99.5 and 99.8% 16S rDNA sequence similarity. Strain Te-2-2T was most closely related to Pseudoalteromonas paragorgicola, with 99.8% 16S rDNA sequence similarity. The DNA G+C base composition was 39.6 to 41.8 mol%, in agreement with other members of the genus Pseudoalteromonas. However, the isolates showed important morphological and physiological differences from previously described species of this genus, with one group forming rod-shaped bacteria typical of Pseudoalteromonas and the other forming vibrioid- to spiral-shaped cells. Based on these differences, and on phylogenetic data, we propose the creation of the new species Pseudoalteromonas telluritireducens sp. nov., with strain Se-1-2-redT (DSMZ=16098T=VKM B-2382T) as the type strain, and Pseudoalteromonas spiralis sp. nov., with strain Te-2-2T (DSMZ=16099T=VKM B-2383T) as the type strain.

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Acknowledgments

This research was funded by grants from the NSERC (Canada) to V.Y. and J.T.B. We thank C.L. van Dover for collection of samples and H. Trüper for assistance with the nomenclature.

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Authors and Affiliations

  1. Department of Microbiology, The University of Manitoba, 422 Buller Building, Winnipeg, MB, Canada, R3T 2N2

    Christopher Rathgeber, Natalia Yurkova & Vladimir Yurkov

  2. DSMZ-Deustche Sammlung von Mikroorganismen und Zellkulturen Gmbh, Mascheroder Weg 1b, D-38124, Braunschweig, Germany

    Erko Stackebrandt & Peter Schumann

  3. Biosciences Electron Microscopy Facility, The University of British Columbia, 517-6270 University Boulevard, Vancouver, BC, Canada, V6T 1Z4

    Elaine Humphrey

  4. Department of Microbiology and Immunology, The University of British Columbia, 300-6174 University Boulevard, Vancouver, BC, Canada, V6T 1Z3

    J. Thomas Beatty

Authors
  1. Christopher Rathgeber
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  2. Natalia Yurkova
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  3. Erko Stackebrandt
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  4. Peter Schumann
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  5. Elaine Humphrey
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  6. J. Thomas Beatty
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  7. Vladimir Yurkov
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Corresponding author

Correspondence to Vladimir Yurkov.

Additional information

The EMBL accession numbers for the 16S rDNA sequences are: Te-1-1, AJ314843; Te-2-2T, AJ314842; Se-1-2-or, AJ314844; Se-1-2-redT, AJ314845.

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Rathgeber, C., Yurkova, N., Stackebrandt, E. et al. Metalloid Reducing Bacteria Isolated from Deep Ocean Hydrothermal Vents of the Juan de Fuca Ridge, Pseudoalteromonas telluritireducens sp. nov. and Pseudoalteromonas spiralis sp. nov. Curr Microbiol 53, 449–456 (2006). https://doi.org/10.1007/s00284-006-0320-2

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