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Nuclear Factor-κB is Activated in Filter-Implanted Vena Cava

  • Laboratory Investigation
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CardioVascular and Interventional Radiology Aims and scope Submit manuscript

Abstract

Background

Implantation of a retrievable vena cava filter (VCF) is an effective method for preventing pulmonary embolism. Retrieval of filters, however, may be difficult due to intimal hyperplasia and inflammation in the cava wall. The transcription factor nuclear factor-kappaB (NF-κB) plays an important role in regulation of numerous genes participating in the inflammatory and proliferative responses of cells. The present study was to determine whether VCF implantation resulted in activation of NF-κB in the venous neointima.

Methods

Filters were placed in vena cava (VC) in four swine for 30 days and then removed. Intimal specimens adhering to the filter struts were analyzed with reference to normal VC tissues. Immunohistochemical analyses were used to assess the NF-κB subunits p65 and p50 and the phosphorylated inhibitor of κB-α (phosphor-IκB-α) in the tissues. NF-κB DNA-binding activity was measured with enzyme-linked immunosorbent assay.

Results

As compared to normal VC tissues, the intimal tissues contained higher percentages of cell nucleus-located p65 and p50, and NF-κB DNA-binding activity. Elevated immunoreactivities of p65, p50 and phosphor-IκB-α were also present in the intima.

Conclusion

The present study demonstrates for the first time that VCF implantation caused NF-κB activation in neointima. We further demonstrate the activation is at least partly due to phosphorylation of IκB-α. Our data suggest that NF-κB activation would significantly contribute to development of intimal hyperplasia and inflammation in filter-inserted vena cava walls. NF-κB might be a therapeutic target for inhibiting filter-induced neointima and improving filter retrieval.

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Acknowledgements

A version of this manuscript was presented at 2017 Annual Meeting of the American Association for the Surgery of Trauma (AAST), September 13–16, 2017, Baltimore, MD.

Funding

This study was supported partly by a grant from the Navicent Health Foundation (Grant Number: 60827116).

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Authors and Affiliations

  1. Department of Surgery, Navicent Health Physician Group, Mercer University School of Medicine and The Medical Center Navicent Health, 1550 College Street, Macon, GA, 31207, USA

    Zhongbiao Wang, Dennis W. Ashley, Lingwei Kong, Don K. Nakayama & Paul S. Dale

  2. Division of Trauma and Critical Care, Navicent Health Physician Group, Mercer University School of Medicine and The Medical Center Navicent Health, 1550 College Street, Macon, GA, 31207, USA

    Zhongbiao Wang & Dennis W. Ashley

  3. Department of Medical Sciences, Mercer University School of Medicine, 1550 College Street, Macon, GA, 31207, USA

    Zhongbiao Wang & Jing Kang

  4. Division of Surgical Oncology, Navicent Health Physician Group, Mercer University School of Medicine and The Medical Center Navicent Health, Macon, GA, 31207, USA

    Paul S. Dale

Authors
  1. Zhongbiao Wang
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  2. Dennis W. Ashley
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  3. Lingwei Kong
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  4. Jing Kang
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  5. Don K. Nakayama
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  6. Paul S. Dale
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Contributions

ZW performed literature search, study design, data interpretation, and writing and critical revision. LK and JK conducted data collection and analysis. DWA, DKN and PSD contributed to critical revision of the manuscript. All provided final approval of the present version for publication.

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Correspondence to Zhongbiao Wang.

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The authors declare that they have no conflict of interest.

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All applicable international, national and/or institutional guidelines for the case and use of animals were followed.

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For this type of study, consent for publication is not required.

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Wang, Z., Ashley, D.W., Kong, L. et al. Nuclear Factor-κB is Activated in Filter-Implanted Vena Cava. Cardiovasc Intervent Radiol 42, 601–607 (2019). https://doi.org/10.1007/s00270-018-2138-2

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  • DOI: https://doi.org/10.1007/s00270-018-2138-2

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