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Insights into the regulatory role of bacterial sncRNA and its extracellular delivery via OMVs

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Abstract

Small noncoding RNAs (sncRNAs) play important regulatory roles in bacterial physiological processes and host-pathogen interactions. Meanwhile, bacterial outer membrane vesicles (OMVs), as naturally secreted outer membrane structures, play a vital role in the interaction between bacteria and their living environment, including the host environment. However, most current studies focus on the biological functions of sncRNAs in bacteria or hosts, while neglecting the roles and regulatory mechanisms of the OMVs that encapsulate these sncRNAs. Therefore, this review aims to summarize the intracellular regulatory roles of bacterial sncRNAs in promoting pathogen survival by regulating virulence, modulating bacterial drug resistance, and regulating iron metabolism, and their extracellular regulatory function for influencing host immunity through host-pathogen interactions. Additionally, we introduce the key role played by OMVs, which serve as important cargoes in bacterial sncRNA–host interactions. We propose emerging pathways of sncRNA action to further discuss the mode of host-pathogen interactions, highlighting that the inhibition of sncRNA delivery by OMVs may prevent the occurrence of infection to some extent. Hence, this review lays the foundation for future prophylactic treatments against bacterial infections and strategies for addressing bacterial drug resistance.

Key points

•sncRNAs have intracellular and extracellular regulatory functions in bacterial physiological processes and host-pathogen interactions.

•OMVs are potential mediators between bacterial sncRNAs and host cells.

•OMVs encapsulating sncRNAs have more potential biological functions.

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Data Availability

Data sharing is not applicable to this review article as no new data were created in this review article.

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Acknowledgements

The authors would like to express their gratitude to Pro. Chengsheng Zhang from The First Affiliated Hospital of Nanchang University for the expert linguistic and academic advice provided.

Funding

This review was supported by the National Natural Science Foundation of China (82203032, 32260193), Training Plan for Academic and Technical Leaders of Major Disciplines in Jiangxi Province-Youth Talent Project (20212BCJ23036), Project for high and talent of Science and Technology Innovation in Jiangxi “double thousand plan” (jxsq2023301110) and the start-up fund from the First Affiliated Hospital of Nanchang University.

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  1. Mengdan He and Shuanshuan Yin contributed equally to this work.

Authors and Affiliations

  1. Center for Molecular Diagnosis and Precision Medicine, The Department of Clinical Laboratory, Jiangxi Provincial Center for Advanced Diagnostic Technology and Precision Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China

    Mengdan He, Shuanshuan Yin, Yi Li, Biaoxian Li, Tian Gong & Qiong Liu

  2. Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China

    Mengdan He, Shuanshuan Yin, Yi Li & Qiong Liu

  3. Gannan Medical University, Ganzhou, 341000, Jiangxi, China

    Xinlei Huang

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MDH, SSY, and QL conceived and wrote the majority of the manuscript. MDH and SSY together to completed abstract and assist in literature search. XLH, YL, and BXL revised the manuscript for its integrity and accuracy. TG and QL approved the final version of this manuscript and take responsibility for its content.

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He, M., Yin, S., Huang, X. et al. Insights into the regulatory role of bacterial sncRNA and its extracellular delivery via OMVs. Appl Microbiol Biotechnol 108, 29 (2024). https://doi.org/10.1007/s00253-023-12855-z

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  • DOI: https://doi.org/10.1007/s00253-023-12855-z

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