Abstract
Vibrio splendidus is a ubiquitous Gram-negative marine bacterium that causes diseases within a wide range of marine cultured animals. Since iron deprivation is the frequent situation that the bacteria usually encounter, we aimed to explore the effect of iron deprivation on the proteomic profile of V. splendidus in the present study. There were 425 differentially expressed proteins (DEPs) responded to the iron deprivation condition. When the cells were grown under iron deprivation condition, the oxidation‒reduction processes, single-organism metabolic processes, the catalytic activity, and binding activity were downregulated, while the transport process, membrane cell component, and ion binding activity were upregulated, apart from the iron uptake processes. Kyoto Encyclopedia of Genes and Genomes analysis showed that various metabolism pathways, biosynthesis pathways, energy generation pathways of tricarboxylic acid cycle, and oxidative phosphorylation were downregulated, while various degradation pathways and several special metabolism pathways were upregulated. The proteomic profiles of cells at a OD600 ≈ 0.4 grown under iron deprivation condition showed high similarity to that of the cells at a OD600 ≈ 0.8 grown without iron chelator 2,2′-bipyridine. Correspondingly, the protease activity, the activity of autoinducer 2 (AI-2), and indole content separately catalyzed by LuxS and TnaA, were measured to verify the proteomic data. Our present study gives basic information on the global protein profiles of V. splendidus grown under iron deprivation condition and suggests that the iron deprivation condition cause the cell growth enter a state of higher cell density earlier.
Key points
• Adaptation of V. splendidus to iron deprivation was explored by proteomic analysis.
• GO and KEGG of DEPs under different iron levels or cell densities were determined.
• Iron deprivation caused the cell enter a state of higher cell density earlier.
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Data availability
All the data generated or analyzed during this study are included in this article. The proteomics data have been deposited into the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX partner repository with the dataset identifier IPX0005175000.
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Funding
This work was finally supported by the National Natural Science Foundation of China (31972833), the Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar (LR20C190001), the Fundamental Research Funds for the Provincial Universities of Zhejiang (SJLZ2020001), the Natural Science Foundation of Ningbo City (2021J062), and the K.C. Wong Magna Fund at Ningbo University.
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HY conducted the experiments, analyzed the data, and wrote the original manuscript. HS conducted parts of the experiments and analyzed the data. JZ conducted parts of the experiments and analyzed parts of the data. WL conducted parts of the experiments and analyzed parts of the data. QH analyzed the data and revised the manuscript. WZ conceived and planned the research, supervised the research, revised the manuscript, and acquired funding.
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Yang, H., Song, H., Zhang, J. et al. Proteomic analysis reveals the adaptation of Vibrio splendidus to an iron deprivation condition. Appl Microbiol Biotechnol 107, 2533–2546 (2023). https://doi.org/10.1007/s00253-023-12460-0
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DOI: https://doi.org/10.1007/s00253-023-12460-0