Abstract
Microbial food spoilage is an important cause of health and economic issues and can occur via resilient contamination of food surfaces. Novel technologies, such as the use of visible light, have seen the light of day to overcome the drawbacks associated with surface disinfection treatments. However, most studies report that photo-inactivation of microorganisms with visible light requires long time treatments. In the present study, a novel light electroluminescent diode (LED)-based device was designed to generate irradiation at an ultra-high power density (901.1 mW/cm2). The efficacy of this technology was investigated with the inactivation of the yeast S. cerevisiae. Short-time treatments (below 10 min) at 405 nm induced a ~4.5 log reduction rate of the cultivable yeast population. The rate of inactivation was positively correlated to the overall energy received by the sample and, at a similar energy, to the power density dispatched by the lamp. A successful disinfection of several food contact surfaces (stainless steel, glass, polypropylene, polyethylene) was achieved as S. cerevisiae was completely inactivated within 5 min of treatments. The disinfection of stainless steel was particularly effective with a complete inactivation of the yeast after 2 min of treatment. This ultra-high irradiance technology could represent a novel cost- and time-effective candidate for microbial inactivation of food surfaces. These treatments could see applications beyond the food industry, in segments such as healthcare or public transport.
Key points
• A novel LED-based device was designed to emit ultra-high irradiance blue light
• Short time treatments induced high rate of inhibition of S. cerevisiae
• Multiple food contact surfaces were entirely disinfected with 5-min treatments
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This project was financially supported by AgroSup Dijon of Université de Bourgogne Franche-Comté, SATT SAYENS (Dijon, France), the European Regional Development Fund (FEDER Alim+) and the National Agency for Research (with GreenDeconta Project ANR18-CE21-0004-01).
Funding
This work was funded by AgroSup Dijon of Université de Bourgogne Franche-Comté, SATT SAYENS (Dijon, France), the European Regional Development Fund (FEDER Alim+) and the National Agency for Research (Grant Number GreenDeconta Project ANR18-CE21-0004-01). The funders had no involvement in study design, data collection, analysis and interpretation.
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LB, SD, and CG conceived and designed research. EL and JA performed experiments. CP developed the mathematical tools. CG, SD, and FC conceived and designed the prototype. EL and TT analyzed data and wrote the manuscript. LB, SD, and CG edited the manuscript. LB and SD supervised the research. All authors read and approved the manuscript.
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Lang, E., Thery, T., Peltier, C. et al. Ultra-high irradiance (UHI) blue light: highlighting the potential of a novel LED-based device for short antifungal treatments of food contact surfaces. Appl Microbiol Biotechnol 106, 415–424 (2022). https://doi.org/10.1007/s00253-021-11718-9
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DOI: https://doi.org/10.1007/s00253-021-11718-9