Abstract
The QuantaMatrix Microfluidic Agarose Channel (QMAC) system was used for rapid drug susceptibility testing (DST). Here, we performed DST using QMAC integrated with the mycobacteria growth indicator tube (MGIT) liquid culture employing a specially designed cross agarose channel for the tuberculosis chip. MGIT-, QMAC-, and Löwenstein–Jensen (LJ)-DSTs were performed using 13 drugs. The protocol for QMAC-DST was optimized using the inoculum obtained after the disaggregation of Mycobacterium tuberculosis clumps in MGIT culture. The completion times of QMAC-DST and MGIT-DST were analyzed, and the results of all three DSTs were compared. Discrepant results were analyzed using line probe assays and DNA sequencing. Nontuberculous mycobacteria were distinguished using the ρ-nitrobenzoic acid inhibition test. The overall agreement rate of QMAT-DST and LJ-DST was 97.0% and that of QMAT-DST and MGIT-DST was 86.3%. An average turnaround time for DST was 5.4 days, which was considerably less than the time required for MGIT-DST. The overall time required to obtain DST results using QMAC-DST integrated with MGIT culture was an average of 18.6 days: 13.2 days for culture and identification and 5.4 days for DST. Hence, QMAC-DST integrated with liquid culture can be used to perform DSTs with short turnaround times and effective detection.
Key points
• QMAC system can simultaneously perform phenotypic DST with 13 anti-TB drugs and PNB.
• An optimized DST protocol led to a marked decrease in clumping in MGIT culture.
• QMAC system integrated with MGIT liquid culture system reduced the turnaround time.
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Acknowledgements
Special thanks to technical members Mr. Seung-Mo Kim, Mrs. So-Yeon Park, and Mr. Jeong Seong Yang.
Funding
This study was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C1567).
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H.K. designed the study, performed the experiments, analyzed the data and protocol, and wrote the manuscript. S. S. revised the manuscript. S.L. drafted the manuscript. E.J., S.K., H.K., and E-G. K. performed the experiments and analyzed the data. S.K. and E-G. K. designed CAT and QMAC systems.
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Sangyeop Lee, EunJi Jo, Haeun Kim, Suyeoun Kim, and Sunghoon Kwon at the time of manuscript submission were employed at QuantaMatrix, Inc., which commercializes the DST technology. They have equity interests in QuantaMatrix, Inc.
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Kim, H., Lee, S., Jo, E. et al. Performance of QuantaMatrix Microfluidic Agarose Channel system integrated with mycobacteria growth indicator tube liquid culture. Appl Microbiol Biotechnol 105, 6059–6072 (2021). https://doi.org/10.1007/s00253-021-11446-0
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DOI: https://doi.org/10.1007/s00253-021-11446-0