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RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking

  • Applied genetics and molecular biotechnology
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Abstract

Glycerol is a key yeast metabolite in winemaking because it contributes to improve the organoleptic properties of wine. It is also a cellular protective molecule that enhances the tolerance of yeasts to osmotic stress and promotes longevity. Thus, its production increases by genetic manipulation, which is of biotechnological and basic interest. Glycerol is produced by diverting glycolytic glyceraldehyde-3-phosphate through the action of glycerol-3-phosphate dehydrogenase (coded by genes GPD1 and GPD2). Here, we demonstrate that RNA-binding protein Pub1p regulates glycerol production by controlling Gpd1p activity. Its deletion does not alter GPD1 mRNA levels, but protein levels and enzymatic activity increase, which explains the higher intracellular glycerol concentration and greater tolerance to osmotic stress of the pub1∆ mutant. PUB1 deletion also enhances the activity of nicotinamidase, a longevity-promoting enzyme. Both enzymatic activities are partially located in peroxisomes, and we detected peroxisome formation during wine fermentation. The role of Pub1p in life span control depends on nutrient conditions and is related with the TOR pathway, and a major connection between RNA metabolism and the nutrient signaling response is established.

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Authors and Affiliations

  1. Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain

    Helena Orozco, Ana Sepúlveda, Cecilia Picazo & Emilia Matallana

  2. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain

    Helena Orozco, Ana Sepúlveda, Cecilia Picazo, Emilia Matallana & Agustín Aranda

Authors
  1. Helena Orozco
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  2. Ana Sepúlveda
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  3. Cecilia Picazo
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  4. Emilia Matallana
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  5. Agustín Aranda
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Correspondence to Helena Orozco.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

This work has been funded by grants from the Spanish Ministry of Science (AGL2011-24,353 and AGL2014-52984R). C.P. has a FPI fellowship.

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The authors have declared that no competing interests exist.

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Orozco, H., Sepúlveda, A., Picazo, C. et al. RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking. Appl Microbiol Biotechnol 100, 5017–5027 (2016). https://doi.org/10.1007/s00253-016-7340-z

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  • DOI: https://doi.org/10.1007/s00253-016-7340-z

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