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Proliferation of diversified clostridial species during biological soil disinfestation incorporated with plant biomass under various conditions

  • Environmental biotechnology
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Abstract

Biological soil disinfestation (BSD) involves the anaerobic decomposition of plant biomass by microbial communities leading to control of plant pathogens. We analyzed bacterial communities in soil of a model experiment of BSD, as affected by biomass incorporation under various conditions, to find out the major anaerobic bacterial groups which emerged after BSD treatments. The soil was treated with Brassica juncea plants, wheat bran, or Avena strigosa plants, irrigated at 20 or 30 % moisture content and incubated at 25–30 °C for 17 days. The population of Fusarium oxysporum f. sp. spinaciae incorporated at the start of the experiment declined markedly for some BSD conditions and rather high concentrations of acetate and butyrate were detected from these BSD-treated soils. The polymerase chain reaction-denaturing gradient gel electrophoresis analysis based on the V3 region of 16S rRNA gene sequences from the soil DNA revealed that bacterial profiles greatly changed according to the treatment conditions. Based on the clone library analysis, phylogenetically diverse clostridial species appeared exceedingly dominant in the bacterial community of BSD soil incorporated with Brassica plants or wheat bran, in which the pathogen was suppressed completely. Species in the class Clostridia such as Clostridium saccharobutylicum, Clostridium acetobutylicum, Clostridium xylanovorans, Oxobacter pfennigii, Clostridium pasteurianum, Clostridium sufflavum, Clostridium cylindrosporum, etc. were commonly recognized as closely related species of the dominant clone groups from these soil samples.

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Acknowledgments

This work was partly supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (development of mitigation and adaptation techniques to global warming in the sectors of agriculture, forestry, and fisheries). We also greatly appreciate the technical assistance of Y. Ohtaki.

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Authors and Affiliations

  1. Faculty of Agriculture, Yamagata University, 1-23, Wakaba-machi, Tsuruoka, 997-8555, Yamagata, Japan

    Subrata Mowlick, Nobuo Kaku, Katsuji Ueki & Atsuko Ueki

  2. NARO Western Region Agricultural Research Center, Hiroshima, Japan

    Toshiaki Takehara

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  1. Subrata Mowlick
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  2. Toshiaki Takehara
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  3. Nobuo Kaku
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  4. Katsuji Ueki
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Correspondence to Atsuko Ueki.

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Mowlick, S., Takehara, T., Kaku, N. et al. Proliferation of diversified clostridial species during biological soil disinfestation incorporated with plant biomass under various conditions. Appl Microbiol Biotechnol 97, 8365–8379 (2013). https://doi.org/10.1007/s00253-012-4532-z

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  • DOI: https://doi.org/10.1007/s00253-012-4532-z

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