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Development of dense microsatellite markers in the entire SLA region and evaluation of their polymorphisms in porcine breeds

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Abstract

We developed 40 microsatellite markers in the entire swine leukocyte antigen (SLA) region, spanning over 2.35 Mb. The average span between markers was 59 kb, and the largest interval between markers was 127 kb. We also evaluated polymorphisms of length for the markers using 97 pigs derived from 12 breeds, including representative commercial breeds. All of the markers were successfully amplified in genomic DNA and shown to be polymorphic. These markers will provide an alternative method for determining the SLA haplotypes instead of direct typing of SLA genes per se. They will be valuable for transplantation studies and for association studies between immunological traits such as disease susceptibility and tumor rejection.

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Fig. 1

Abbreviations

HT:

Heterozygosity

MHC:

Major histocompatibility complex

MS:

Microsatellite

PCR:

Polymerase chain reaction

PIC:

Polymorphic information content

SLA:

Swine leukocyte antigen

SSC:

Swine chromosome

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Acknowledgements

Pig DNA samples were kindly provided by the National Livestock Breeding Center and by Kagoshima, Shizuoka and Tokushima Prefectures in Japan. This work was supported by a Grant-in-Aid from the Japan Racing Association and by the Animal Genome Research Project of the Ministry of Agriculture, Forestry, and Fisheries, Japan.

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Authors and Affiliations

  1. Second Research Division, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan

    Maiko Tanaka, Michiko Domukai & Naohiko Okumura

  2. Animal Genome Research Program (NIAS/STAFF), 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan

    Maiko Tanaka, Michiko Domukai, Naohiko Okumura, Takashi Awata & Hirohide Uenishi

  3. Department of Molecular Life Science, Division of Basic Molecular Science and Molecular Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, 259-1193, Japan

    Asako Ando

  4. Laboratoire Mixte de Radiobiologie et d'Etude du Génome, Institut National de la Recherche Agronomique et Commissariat à l'Energie Atomique, Domaine de Vilvert, 78352 Jouy-en-Josas, Cedex, France

    Christine Renard & Patrick Chardon

  5. Animal Genome Laboratory, Genome Research Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Takashi Awata & Hirohide Uenishi

Authors
  1. Maiko Tanaka
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  2. Asako Ando
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  3. Christine Renard
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  4. Patrick Chardon
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  5. Michiko Domukai
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  6. Naohiko Okumura
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  7. Takashi Awata
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  8. Hirohide Uenishi
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Correspondence to Hirohide Uenishi.

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Tanaka, M., Ando, A., Renard, C. et al. Development of dense microsatellite markers in the entire SLA region and evaluation of their polymorphisms in porcine breeds. Immunogenetics 57, 690–696 (2005). https://doi.org/10.1007/s00251-005-0037-6

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  • DOI: https://doi.org/10.1007/s00251-005-0037-6

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